Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Periodontics, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
BMC Oral Health. 2022 Dec 1;22(1):551. doi: 10.1186/s12903-022-02602-7.
Suppressor of cytokine signalling (SOCS) family comprises a group of proteins that impede JAK/STAT signalling, thus being involved in the pathogenesis of immune-related conditions. In the present work, we aimed at identification of the role of SOCS genes in the pathogenesis of periodontitis through evaluation of their expression levels both in the circulation and in the affected tissues of patients. Thus, we measured expression levels of SOCS1-3 and SOCS5 transcripts in the blood and gingival samples of patients with periodontitis in comparison with control samples obtained during dental crown lengthening. Expressions of SOCS1, SOCS2, SOCS3 and SOCS5 genes were similar between gingival tissues of patients and controls. However, our results demonstrated under-expression of SOCS1 in blood samples of patients compared with controls (Ratio of mean expression (RME) = 0.47, P value = 0.04). The same pattern was observed among female subjects (RME = 0.38, P value = 0.04). SOCS2 was down-regulated in blood samples of female patients compared with female controls (RME = 0.22, P value = 0.04). SOCS3 was also under-expressed in the circulation of total cases versus total controls (RME = 0.29, P value = 0.02) and in female patients compared with female controls (RME = 0.19, P value = 0.04). Expression of SOCS5 was not different between blood samples two study groups. SOCS2 had the best function in separation of affected tissues from unaffected ones (AUC = 0.66, sensitivity = 0.39, specificity = 0.83). SOCS3 was superior to other transcripts in differentiation of blood samples of patients from normal blood samples (AUC = 0.69, sensitivity = 0.81, specificity = 0.68). Combination of transcript levels of SOCS1, SOCS2, SOCS3 and SOCS5 genes enhanced the AUC values to 0.64 and 0.67 in tissue and blood specimens, respectively. Taken together, certain SOCS genes have been found to be dysregulated in the circulation of patients with periodontitis.
抑制细胞因子信号转导（SOCS）家族由一组蛋白质组成，这些蛋白质可阻碍 JAK/STAT 信号转导，从而参与免疫相关疾病的发病机制。在本工作中，我们旨在通过评估 SOCS 基因在患者循环和受累组织中的表达水平，来确定 SOCS 基因在牙周炎发病机制中的作用。因此，我们比较了牙周炎患者和牙冠延长术获得的对照组的血液和牙龈样本中 SOCS1-3 和 SOCS5 转录本的表达水平。SOCS1、SOCS2、SOCS3 和 SOCS5 基因在患者和对照组的牙龈组织中表达相似。然而，我们的结果表明，与对照组相比，患者血液中 SOCS1 的表达下调（平均表达比（RME）= 0.47，P 值= 0.04）。在女性患者中也观察到相同的模式（RME= 0.38，P 值= 0.04）。与女性对照组相比，女性患者的血液中 SOCS2 下调（RME= 0.22，P 值= 0.04）。SOCS3 在总病例与总对照组的循环中也表达不足（RME= 0.29，P 值= 0.02），在女性患者与女性对照组相比时（RME= 0.19，P 值= 0.04）。两组研究对象的血液样本中 SOCS5 的表达无差异。SOCS2 在区分病变组织和非病变组织方面具有最佳功能（AUC= 0.66，敏感性= 0.39，特异性= 0.83）。与其他转录本相比，SOCS3 更能区分患者血液样本和正常血液样本（AUC= 0.69，敏感性= 0.81，特异性= 0.68）。SOCS1、SOCS2、SOCS3 和 SOCS5 基因转录本水平的组合可将 AUC 值分别提高到组织和血液标本中的 0.64 和 0.67。综上所述，某些 SOCS 基因在牙周炎患者的循环中出现失调。
