Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Front Immunol. 2022 Nov 15;13:1023943. doi: 10.3389/fimmu.2022.1023943. eCollection 2022.
Broadly neutralizing ability is critical for developing the next-generation SARS-CoV-2 vaccine. We collected sera samples between December 2021-January 2022 from 113 Taiwan naïve participants after their second dose of homologous vaccine (AZD1222, mRNA-1273, BNT162-b2, and MVC-COV1901) and compared the differences in serological responses of various SARS-CoV-2 vaccines. Compared to AZD1222, the two mRNA vaccines could elicit a higher level of anti-S1-RBD binding antibodies with higher broadly neutralizing ability evaluated using pseudoviruses of various SARS-CoV-2 lineages. The antigenic maps produced from the neutralization data implied that Omicron represents very different antigenic characteristics from the ancestral lineage. These results suggested that constantly administering the vaccine with ancestral Wuhan spike is insufficient for the Omicron outbreak. In addition, we found that anti-ACE2 autoantibodies were significantly increased in all four vaccinated groups compared to the unvaccinated pre-pandemic group, which needed to be investigated in the future.
广泛中和能力对于开发下一代 SARS-CoV-2 疫苗至关重要。我们于 2021 年 12 月至 2022 年 1 月期间收集了 113 名台湾未接种疫苗的参与者在接种第二剂同源疫苗（AZD1222、mRNA-1273、BNT162-b2 和 MVC-COV1901）后的血清样本，并比较了各种 SARS-CoV-2 疫苗的血清学反应差异。与 AZD1222 相比，两种 mRNA 疫苗可以诱导更高水平的抗 S1-RBD 结合抗体，并且使用各种 SARS-CoV-2 谱系的假病毒评估具有更高的广泛中和能力。从中和数据生成的抗原图谱表明，Omicron 与原始谱系代表非常不同的抗原特征。这些结果表明，对于 Omicron 爆发，仅用原始武汉刺突施打疫苗是不够的。此外，我们发现与未接种大流行前组相比，所有四个接种组的抗 ACE2 自身抗体均显著增加，这需要在未来进行研究。
