Promoted Spore Formation of fmbJ by its Secondary Metabolite Bacillomycin D Coordinated with Mn.

UNLABELLED

In , the spore formation process is associated with the synthesis and release of secondary metabolites. A large number of studies have been conducted to systematically elucidate the pathways and mechanisms of spore formation. However, there are no studies have explored the relationship between secondary metabolites and spores. In this study, we investigated the relationship between its secondary metabolite bacillomycin D (BD) and spores using the simpler dipicolonic acid fluorimetry assay for spore counting in fmbJ. Our results showed that BD could promote the spore formation of fmbJ and had a synergistic effect with certain concentrations of Mn. When 15.6 mg/L of BD and 1 mM of Mn were added, the number of fmbJ spores increased from 1.42 × 10 CFU/mL to 2.02 × 10 CFU/mL after 36 h of incubation. The expressions of spore formation (, , , , and ) and Mn-related genes (, , , ) were studied by RT-PCR. The results indicated that BD and Mn promoted the spore formation of fmbJ by stimulating the transcription of , and increasing the influence of - phosphorylation transmission. This study provided a new idea to improve the spore production of and laid the foundation for its industrial production.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12088-022-01026-9.