Batra Parichaya, Das Sushma, Patel Poonam
The Department of Periodontics, The Oxford Dental College, Bommanahalli, Bangalore, India.
The Department of Public Health Dentistry, Vydehi Institute of Dental Sciences & Research Centre, No. 82, EPIP Area, Nallurahalli, Whitefield, Bangalore 560066, India.
Saudi Dent J. 2019 Jul;31(3):316-321. doi: 10.1016/j.sdentj.2019.03.010. Epub 2019 Mar 27.
Proinflammatory cytokines appear to have a central role in the destruction of periodontal tissues. By binding to Receptor Activator of Nuclear Factor κb (RANK) on osteoclast progenitor cells, these cytokines are locally responsible for the activation of bone resorbing osteoclasts differentiation and function. Interleukin-34 (IL-34) is a proinflammatory cytokine derived from the osteoblasts and plays an important role in osteoclastogenesis. The present study was carried out to assess the levels of IL-34 in Gingival Crevicular Fluid (GCF) in periodontally healthy patients, chronic periodontitis and aggressive periodontitis.
A total of 90 patients were recruited in the study and were divided into 3 groups: Periodontally healthy patients (Group I), Chronic periodontitis (Group II) and Aggressive periodontitis (Group III). Clinical Parameters like plaque index, gingival index, probing depth, clinical attachment levels were recorded and GCF samples were collected at baseline for the assessment of IL-34 levels in all the three groups. IL-34 levels were calculated using Enzyme-Linked Immune-Sorbent Assay (ELISA).
The mean GCF levels of IL-34 in Group I was 47.22 ± 18.04 pg/ml, Group II was 103.76 ± 26.61 pg/ml and in Group III was 191.71 ± 49.24 pg/ml. The mean GCF IL-34 level was found to be higher in Group III followed by Group II and Group I. The IL-34 levels correlated with clinical parameters like plaque index, gingival index, probing depth, clinical attachment levels. But correlation with probing depth and clinical attachment level were significantly higher in Group III followed by Group II and Group I.
The study demonstrated higher levels of IL-34 in aggressive periodontitis when compared with chronic periodontitis. IL-34, a novel diagnostic marker which was found to be at higher level in GCF of aggressive and chronic periodontitis patients.
促炎细胞因子似乎在牙周组织破坏中起核心作用。通过与破骨细胞前体细胞上的核因子κB受体激活剂（RANK）结合，这些细胞因子在局部负责激活骨吸收破骨细胞的分化和功能。白细胞介素-34（IL-34）是一种源自成骨细胞的促炎细胞因子，在破骨细胞生成中起重要作用。本研究旨在评估牙周健康患者、慢性牙周炎患者和侵袭性牙周炎患者龈沟液（GCF）中IL-34的水平。
本研究共招募了90名患者，分为3组：牙周健康患者（第一组）、慢性牙周炎患者（第二组）和侵袭性牙周炎患者（第三组）。记录菌斑指数、牙龈指数、探诊深度、临床附着水平等临床参数，并在基线时收集GCF样本，以评估三组患者的IL-34水平。使用酶联免疫吸附测定法（ELISA）计算IL-34水平。
第一组GCF中IL-34的平均水平为47.22±18.04 pg/ml，第二组为103.76±26.61 pg/ml，第三组为191.71±49.24 pg/ml。发现第三组GCF中IL-34的平均水平最高，其次是第二组和第一组。IL-34水平与菌斑指数、牙龈指数、探诊深度、临床附着水平等临床参数相关。但第三组与探诊深度和临床附着水平的相关性明显高于第二组和第一组。
该研究表明，与慢性牙周炎相比，侵袭性牙周炎患者的IL-34水平更高。IL-34是一种新型诊断标志物，在侵袭性和慢性牙周炎患者的GCF中含量较高。
