虚拟分子印迹固相萃取-表面增强拉曼光谱法测定花生中的黄曲霉毒素B1
Dummy molecularly imprinted solid-phase extraction-SERS determination of AFB1 in peanut.
作者信息
Fan Li, Zhang Qiong, Wang Feng, Yang Haifeng
机构信息
The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China.
The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, and Department of Chemistry, Shanghai Normal University, Shanghai 200234, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Mar 5;288:122130. doi: 10.1016/j.saa.2022.122130. Epub 2022 Nov 28.
As a class I carcinogen, aflatoxin B1 (AFB1) contamination in foods and feeds accounts for 75 % of the total mycotoxin contamination. In this work, a simple and reliable surface-enhanced Raman spectroscopy (SERS) method for sensitive and selective detection of AFB1 in peanut samples integrated with dummy molecularly imprinted polymers (DMIPs) is developed. N-isopropylacrylamide (NIPAM) and 7-ethoxycoumarin (7-EOC) are chosen as monomer and dummy template, respectively and their ratio was screened through molecular design in both of kinetic and static adsorption views to form the optimal DMIPs. As-prepared dummy molecularly imprinted solid-phase extraction (DMISPE) could selectively enrich AFB1 from peanut samples. Finally, a liquid-liquid interface self-assembly constructed thioctic acid-decorated AgNPs monolayer film (TA-AgNPs MF) as a SERS-active substrate is employed to determine the amount of AFB1 eluted from DMISPE. SERS assay shows high detection sensitivity for AFB1 in peanut samples with limit of detection of 0.1 μg L and a linear concentration relationship range from 0.1 to 10 μg L.
作为一种一类致癌物，食品和饲料中的黄曲霉毒素B1（AFB1）污染占霉菌毒素总污染的75%。在本研究中，开发了一种简单可靠的表面增强拉曼光谱（SERS）方法，用于结合虚拟分子印迹聚合物（DMIPs）对花生样品中的AFB1进行灵敏且选择性的检测。分别选择N-异丙基丙烯酰胺（NIPAM）和7-乙氧基香豆素（7-EOC）作为单体和虚拟模板，并通过动力学和静态吸附视角的分子设计筛选它们的比例，以形成最优的DMIPs。所制备的虚拟分子印迹固相萃取（DMISPE）能够从花生样品中选择性富集AFB1。最后，采用液-液界面自组装构建的硫辛酸修饰的AgNPs单层膜（TA-AgNPs MF）作为SERS活性基底，来测定从DMISPE洗脱的AFB1的量。SERS分析对花生样品中的AFB1显示出高检测灵敏度，检测限为0.1 μg/L，线性浓度关系范围为0.1至10 μg/L。
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