Huang Bo, Jiang Yixuan, Zhang Liang, Yang Bo, Guo Yanjun, Yang Xingmei, Gong Ping
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Department of General Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Tissue Cell. 2023 Feb;80:101985. doi: 10.1016/j.tice.2022.101985. Epub 2022 Nov 21.
Schwann cells (SCs) are the major component of myelin sheath in the peripheral nervous system, which are necessary in the development, function maintenance, and repair of peripheral nerves. This study aimed to investigate the potential mechanism of low-intensity pulsed ultrasound (LIPUS) affecting the proliferation and myelinating activity of SCs. Rat Schwann cell line RSC96 were cultured and exposed to LIPUS of different duty ratios (control, 20 %, 50 %, 80 %). Results demonstrated that LIPUS with a duty ratio of 50 % showing the maximal effect in facilitating proliferation of SCs. The expressions of Krox20 and myelin basic protein (MBP), the key molecules of SC myelination, and the potent inducer of myelination neuregulin 1 (NRG1) and its receptors ErbB2 and ErbB3 increased significantly by LIPUS. The reaction of these factors to LIPUS were both time- and duty ratio-dependent: namely LIPUS with higher duty ratios took effects when applied repeatedly over more consecutive days. These observations indicated that NRG1/ErbB signaling pathway might contribute to the effects of LIPUS on the proliferation and myelinating status of SCs, which could be one of the mechanisms in the protective role of LIPUS in nerve repair and regeneration. Our work provided novel insights for promising strategies of nerve repair therapy.
施万细胞（SCs）是周围神经系统中髓鞘的主要组成部分，对周围神经的发育、功能维持和修复至关重要。本研究旨在探讨低强度脉冲超声（LIPUS）影响施万细胞增殖和髓鞘形成活性的潜在机制。培养大鼠施万细胞系RSC96，并将其暴露于不同占空比的LIPUS（对照组、20%、50%、80%）下。结果表明，占空比为50%的LIPUS在促进施万细胞增殖方面显示出最大效果。LIPUS显著增加了施万细胞髓鞘形成的关键分子Krox20和髓鞘碱性蛋白（MBP），以及髓鞘形成的强效诱导因子神经调节蛋白1（NRG1）及其受体ErbB2和ErbB3的表达。这些因子对LIPUS的反应均具有时间和占空比依赖性：即占空比越高的LIPUS在连续多日重复应用时才会产生效果。这些观察结果表明，NRG1/ErbB信号通路可能参与了LIPUS对施万细胞增殖和髓鞘形成状态的影响，这可能是LIPUS在神经修复和再生中发挥保护作用的机制之一。我们的工作为有前景的神经修复治疗策略提供了新的见解。
