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用于多模态光声显微镜和光相干断层扫描增强分子成像的链状金纳米颗粒簇。

Chain-like gold nanoparticle clusters for multimodal photoacoustic microscopy and optical coherence tomography enhanced molecular imaging.

机构信息

Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI, 48105, USA.

NTT-Hitech Institutes, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam.

出版信息

Nat Commun. 2021 Jan 4;12(1):34. doi: 10.1038/s41467-020-20276-z.

DOI:10.1038/s41467-020-20276-z
PMID:33397947
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7782787/
Abstract

Colloidal gold nanoparticles (GNPs) serve as promising contrast agents in photoacoustic (PA) imaging, yet their utility is limited due to their absorption peak in the visible window overlapping with that of hemoglobin. To overcome such limitation, this report describes an ultrapure chain-like gold nanoparticle (CGNP) clusters with a redshift peak wavelength at 650 nm. The synthesized CGNP show an excellent biocompatibility and photostability. These nanoparticles are conjugated with arginine-glycine-aspartic acid (RGD) peptides (CGNP clusters-RGD) and validated in 12 living rabbits to perform multimodal photoacoustic microscopy (PAM) and optical coherence tomography (OCT) for visualization of newly developed blood vessels in the sub-retinal pigment epithelium (RPE) space of the retina, named choroidal neovascularization (CNV). The PAM system can achieve a 3D PAM image via a raster scan of 256 × 256 pixels within a time duration of 65 s. Intravenous injection of CGNP clusters-RGD bound to CNV and resulted in up to a 17-fold increase in PAM signal and 176% increase in OCT signal. Histology indicates that CGNP clusters could disassemble, which may facilitate its clearance from the body.

摘要

胶体金纳米粒子 (GNPs) 可用作光声 (PA) 成像的有前途的对比剂，但由于其吸收峰在可见光窗口与血红蛋白重叠，因此其用途有限。为了克服这种限制，本报告描述了一种具有 650nm 红移峰波长的超纯链状金纳米粒子 (CGNP) 簇。合成的 CGNP 表现出优异的生物相容性和光稳定性。这些纳米粒子与精氨酸-甘氨酸-天冬氨酸 (RGD) 肽 (CGNP 簇-RGD) 缀合，并在 12 只活兔中进行了多模态光声显微镜 (PAM) 和光学相干断层扫描 (OCT) 验证，以可视化视网膜色素上皮 (RPE) 下空间中新开发的血管，称为脉络膜新生血管 (CNV)。PAM 系统可以通过在 65s 内对 256×256 像素进行光栅扫描来实现 3D PAM 图像。CGNP 簇-RGD 与 CNV 结合后静脉注射，导致 PAM 信号增加 17 倍，OCT 信号增加 176%。组织学表明 CGNP 簇可以解体，这可能有助于其从体内清除。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/fa344c9e93cb/41467_2020_20276_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/64721abea90e/41467_2020_20276_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/1322acbcc588/41467_2020_20276_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/6c2f64bd39aa/41467_2020_20276_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/969e71869a60/41467_2020_20276_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/04d2ac005a80/41467_2020_20276_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/42180a41d511/41467_2020_20276_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/1497b84f5b5b/41467_2020_20276_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/fa344c9e93cb/41467_2020_20276_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/64721abea90e/41467_2020_20276_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/1322acbcc588/41467_2020_20276_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/6c2f64bd39aa/41467_2020_20276_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/969e71869a60/41467_2020_20276_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/04d2ac005a80/41467_2020_20276_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/42180a41d511/41467_2020_20276_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/1497b84f5b5b/41467_2020_20276_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e48f/7782787/fa344c9e93cb/41467_2020_20276_Fig8_HTML.jpg

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