Liu Hengyu, Nan Zhezhu, Zhu Hongrui, Chen Junjie, Ilovitsh Tali, Wu Daocheng, Wan Mingxi, Feng Yi
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, People's Republic of China.
Department of Biomedical Engineering, Tel Aviv University, 6997801, Tel Aviv, Israel.
Mikrochim Acta. 2022 Dec 7;190(1):14. doi: 10.1007/s00604-022-05583-0.
Quantitative detection of different types of reactive oxygen species (ROS) is vital for understanding the crucial roles of them in biological processes. However, few researches achieved the detection of multiple types of ROS with one probe until now. Given this, we designed and prepared fluorescent gold nanoclusters capped by dual ligand bovine serum albumin and lysozyme (BSA-LYS-AuNCs), which could detect 3 specific types of ROS based on its different fluorescent responses to HO, •OH and ClO, respectively. The limit of detection (LOD) of HO, •OH, and ClO was as low as 0.82 μM, 0.45 μM, and 0.62 μM. Moreover, as an important ROS type, ClO was detected with high sensitivity and low LOD by BSA-LYS-AuNCs. It was also proved that the crosslinking of protein mainly contributed to the unique fluorescent characteristics of the probe exposing to ClO. Furthermore, the fluorescent probe achieved the smart detection of hROS (including •OH and ClO) and wROS (the form of HO) in the real sample, which could also been applied specifically to the detection of antioxidants, e.g. ascorbic acid. The gold nanoclusters developed have high potential for the smart detection of multiple ROS in the body fluid of organisms.
