Lee Ji Su, Lee Seon Mi, Son Hyung Seok, Yoon Yi Na, Shin Jung Eun, Sul Woo Jun, Yu Da Ae, Choe Yong Beom, Lee Yang Won
Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
Department of Systemic Biotechnology, Chung-Ang University, Anseong, Korea.
Ann Dermatol. 2022 Dec;34(6):461-471. doi: 10.5021/ad.22.153.
Recently, microbiome research has been actively conducted for various skin areas. However, no study has yet compared the microbiome of bacteria and fungi in the ear canal of healthy individuals and patients with chronic otitis externa in Korea.
This study aimed to investigate the difference in the distribution of fungal and bacterial microbial communities in ear canal samples of healthy individuals and patients with chronic otitis externa.
In 24 patients with bilateral chronic otitis externa and 24 healthy controls, cotton swabs were used to obtain samples from the bilateral ear canal. To characterize the fungal and bacterial communities, we sequenced and analyzed the 16S rRNA V4-V5 and ITS1 regions using Quantitative Insights into Microbial Ecology 2, respectively.
The alpha diversity analysis for bacteria and fungi confirmed that both richness and evenness decreased in the patient group. The beta diversity analysis for bacteria confirmed that these parameters differed between the control and patient groups. The beta diversity analysis for fungi showed no difference between the groups.
We observed different skin microbiomes in the patients with chronic otitis externa compared with those in the healthy individuals.
最近，针对不同皮肤区域积极开展了微生物组研究。然而，韩国尚未有研究比较健康个体和慢性外耳道炎患者耳道内细菌和真菌的微生物组。
本研究旨在调查健康个体和慢性外耳道炎患者耳道样本中真菌和细菌微生物群落分布的差异。
在24例双侧慢性外耳道炎患者和24例健康对照中，使用棉拭子从双侧耳道获取样本。为了表征真菌和细菌群落，我们分别使用微生物生态学定量洞察2对16S rRNA V4-V5和ITS1区域进行测序和分析。
细菌和真菌的α多样性分析证实，患者组的丰富度和均匀度均降低。细菌的β多样性分析证实，这些参数在对照组和患者组之间存在差异。真菌的β多样性分析显示两组之间没有差异。
我们观察到慢性外耳道炎患者与健康个体的皮肤微生物组不同。
