Shijiazhuang Railway Institute for Disease Control and Prevention, China Railway Beijing Group Co.,Ltd., Shijiazhuang, Hebei, China.
Department of Bone Oncology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Medicine (Baltimore). 2022 Dec 2;101(48):e31550. doi: 10.1097/MD.0000000000031550.
Flow cytometry is a classical method for analyzing human peripheral blood lymphocyte subsets. This study aims to explore a new combination of antibody and labeled fluorescein for detecting lymphocyte subsets by comparing the effects of different combinations of antibody and labeled fluorescein in flow cytometry. We conducted a prospective study and enrolled 362 healthy patients undergoing physical examination in the medical examination center of the third hospital of hebei medical university. Venous blood was drawn from volunteers at the same time in the morning and divided into 3 tubes (Tube A, Tube B and Tube C). T lymphocytes were detected by 3-colors method (CD4-FITC/CD8-PE/CD3-PC5) in Tube A, B lymphocytes were detected by 2-colors method (CD19-FITC/CD3-PE) in Tube B, and T lymphocytes and B lymphocytes were detected by 4-colors method (CD4-FITC/CD8-PE/CD3-PC5/CD19-FITC) in Tube C. The repeatability and accuracy of the test scheme for Tube C shall not be inferior to that of Tube A and Tube B. There were no significant difference in the results of CD3 + and CD4+/CD8 + between Tube A and C, as well as in the results of CD3 + and CD19 + between Tube B and C. Pearson correlation analysis showed that the test results of a and C and B and C were highly correlated. The 4-colors method (CD4-FITC/CD8-PE/CD3-PC5/CD19-FITC) can detect T lymphocytes and B lymphocytes at the same time, reduce the use of fluorescence channels and save the detection cost, which is worthy of recommendation.
流式细胞术是分析人外周血淋巴细胞亚群的经典方法。本研究旨在通过比较不同抗体和标记荧光素组合在流式细胞术中的效果，探索一种新的抗体和标记荧光素组合，用于检测淋巴细胞亚群。我们进行了一项前瞻性研究，纳入了河北医科大学第三医院体检中心 362 名健康体检者。志愿者于早晨同时抽取静脉血，分为 3 管（管 A、管 B 和管 C）。管 A 采用三色法（CD4-FITC/CD8-PE/CD3-PC5）检测 T 淋巴细胞，管 B 采用双色法（CD19-FITC/CD3-PE）检测 B 淋巴细胞，管 C 采用四色法（CD4-FITC/CD8-PE/CD3-PC5/CD19-FITC）同时检测 T 淋巴细胞和 B 淋巴细胞。管 C 的试验方案的重复性和准确性不应低于管 A 和管 B。管 A 和管 C 之间 CD3+和 CD4+/CD8+的结果以及管 B 和管 C 之间 CD3+和 CD19+的结果无显著差异。Pearson 相关分析显示，管 A 和管 C 以及管 B 和管 C 的检测结果高度相关。四色法（CD4-FITC/CD8-PE/CD3-PC5/CD19-FITC）可同时检测 T 淋巴细胞和 B 淋巴细胞，减少荧光通道的使用，节约检测成本，值得推荐。
