哥伦比亚野生巴比拉（）原位分离菌株的分子特征分析 。 需注意，原文中“spp.”和“Babilla ()”部分信息不完整，可能影响准确理解。
Molecular Characterization of spp. Isolates from Wild Colombian Babilla () Isolated In Situ.
作者信息
Rodríguez-Hernández Roy, Herrera-Sánchez María Paula, Ortiz-Muñoz Julián David, Mora-Rivera Cristina, Rondón-Barragán Iang Schroniltgen
机构信息
Poultry Research Group, Faculty of Veterinary Medicine, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia.
Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia.
出版信息
Animals (Basel). 2022 Nov 30;12(23):3359. doi: 10.3390/ani12233359.
enterica is a pathogen capable of colonizing various environments, including the intestinal tract of different animals such as mammals, birds, and reptiles, which can act as carriers. infection induces different clinical diseases, gastroenteritis being the most common, which in some cases, can evolve to septicemia and meningitis. Reptiles and amphibians have been reported as a reservoir of , and transmission of the pathogen to humans has been documented. This study aimed to determine the presence of virulence genes and characterize the genotypic antibiotic resistance profile in strains isolated from obtained in situ (natural habitat) in Prado, Tolima, Colombia in a previous study and stored in a strain bank in our laboratory. Fifteen strains were evaluated through endpoint PCR to determine the presence of resistance genes and virulence genes. The genes , , and were detected in all the strains that confer resistance to ampicillin, streptomycin, and sulfamethoxazole, as well as the virulence genes , , , , , , , , , , , , , , , , , , and , indicating the possible role of babilla () as a carrier of multidrug-resistant bacteria.
肠炎沙门氏菌是一种能够在多种环境中定殖的病原体，包括不同动物（如哺乳动物、鸟类和爬行动物）的肠道，这些动物可作为携带者。感染会引发不同的临床疾病，其中肠胃炎最为常见，在某些情况下，还可能发展为败血症和脑膜炎。据报道，爬行动物和两栖动物是肠炎沙门氏菌的储存宿主，并且该病原体向人类的传播已有记录。本研究旨在确定从哥伦比亚托利马省普拉多市原位（自然栖息地）采集并保存在我们实验室菌株库中的菌株中，毒力基因的存在情况，并对其基因型抗生素耐药谱进行表征。通过终点PCR对15株肠炎沙门氏菌进行评估，以确定耐药基因和毒力基因的存在情况。在所有对氨苄青霉素、链霉素和磺胺甲恶唑具有抗性的菌株中均检测到了blaTEM、aadA1和sul1基因，以及毒力基因spvC、spvR、spvB、spvA、mgtC、pipB2、ssaV、sseI、sipB、sopE、sopE2、gogB、misL、mgtA、hilA、invA、prgH、spaO、sseA、orgA、msgA、pagC、sopB、sifA、sifB、mksB、stm4483、stm4484、stm4485、stm4486、stm4487、stm4488、stm4489、stm4490、stm4491、stm4492、stm4493、stm4494、stm4495、stm4496、stm4497、stm4498、stm4499、stm4500、stm4501、stm4502、stm4503、stm4504、stm4505、stm4506、stm4507、stm4508、stm4509、stm4510、stm4511、stm4512、stm4513、stm4514、stm4515、stm4516、stm4517、stm4518、stm4519、stm4520、stm4521、stm4522、stm4523、stm4524、stm4525、stm4526、stm4527、stm4528、stm4529、stm4530、stm
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