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从家禽和人类样本中分离出的毒力因子的分子检测

Molecular Detection of Virulence Factors in Isolated from Poultry and Human Samples.

作者信息

Lozano-Villegas Kelly Johanna, Herrera-Sánchez María Paula, Beltrán-Martínez Mónica Alexandra, Cárdenas-Moscoso Stefany, Rondón-Barragán Iang Schroniltgen

机构信息

Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué, Tolima, Colombia.

Immunobiology and Pathogenesis Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, A. A 546, Ibagué 730006299, Colombia.

出版信息

Vet Med Int. 2023 Mar 2;2023:1875253. doi: 10.1155/2023/1875253. eCollection 2023.

Abstract

Salmonellosis is a common infectious disease in humans caused by spp., which in recent years has shown an increase in its incidence, with products of avian origin being a common source of transmission. To present a successful infective cycle, there are molecular mechanisms such as virulence factors that provide characteristics that facilitate survival, colonization, and damage to the host. According to this, the study aims to characterize the virulence factors of spp. strains isolated from broilers ( = 39) and humans ( = 10). The presence of 24 virulence genes was evaluated using end-point PCR. All the strains of spp. isolated from broiler chickens revealed presence of 7/24 (29, 16%) virulence genes (, , , , , , and ). Regarding the strains isolated from cases of gastroenteritis in humans, all strains contained (14/24, 58, 33%) virulence genes (, , , , , , , , , , , , , and ). In summary, the presence of virulence genes in different strains of isolated from broilers and humans could be described as bacteria with potential pathogenicity due to the type and number of virulence genes detected. These findings are beneficial for the pathogenic monitoring of in Colombia.

摘要

沙门氏菌病是一种由沙门氏菌属引起的常见人类传染病,近年来其发病率呈上升趋势,禽类来源产品是常见的传播源。为了呈现一个成功的感染周期,存在一些分子机制,如毒力因子,这些因子赋予细菌有助于在宿主体内生存、定殖和造成损害的特性。据此,本研究旨在对从肉鸡(n = 39)和人类(n = 10)中分离出的沙门氏菌属菌株的毒力因子进行表征。使用终点PCR评估24个毒力基因的存在情况。从肉鸡中分离出的所有沙门氏菌属菌株均显示存在7/24(29.16%)个毒力基因(spvC、spvR、spvB、spvA、hilA、invA和sipA)。对于从人类肠胃炎病例中分离出的菌株,所有菌株均含有14/24(58.33%)个毒力基因(spvC、spvR、spvB、spvA、hilA、invA、sipA、pefA、sefA-D、mgtC、ssaV、stm2168、sseJ和spiC)。总之,由于检测到的毒力基因的类型和数量,从肉鸡和人类中分离出的不同沙门氏菌菌株中存在毒力基因可被描述为具有潜在致病性的细菌。这些发现有助于哥伦比亚对沙门氏菌的致病性监测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d5e/9998162/d7acec92ec3d/VMI2023-1875253.001.jpg

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