Filippone Alessia, Ardizzone Alessio, Bova Valentina, Lanza Marika, Casili Giovanna, Cuzzocrea Salvatore, Esposito Emanuela, Campolo Michela, Paterniti Irene
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D' Alcontres, 31, 98166 Messina, Italy.
J Clin Med. 2022 Nov 29;11(23):7073. doi: 10.3390/jcm11237073.
Irritable Bowel Syndrome is a gastrointestinal disorder that affects the large intestine, which encompasses several symptoms including, but not limited to, abdominal pain, bloating and dysmotility. In particular, IBS associated with constipation (IBS-C) is characterized by hard and dry stools and inadequate evacuation and difficulty in defecation. Although several drugs ameliorate intestinal modifications and constipation-associated features, management of IBS is still a challenge. Natural compounds including Xyloglucan and pea protein (XP) and Chia seed powder (CS) are widely known to possess beneficial effects in counteracting several gastrointestinal disorders. Here, we aimed to assess the combined effects of XP and CS to treat constipation-related alterations in an IBS-C rat model. IBS-C was induced by gastric instillation of 2 mL of cold water (0-4 °C) for 14 days and Xiloglucan, Pea protein and Chia seeds (XP + CS) treatment was orally administered for 7 days. On day 22, colon tissues were collected for histological analysis. Our results showed that XP + CS administration attenuated constipation-related parameters by increasing body weight and food and water intake. Upon XP + CS treatment, from day 14 to 22, stool moisture content was restored to physiological level. Colonic tissues from IBS-C rats depicted a disruption of the organ architecture accompanied by edema. Loss of colonic structure was reflected by the marked reduction of tight junction protein expression, Occludin and zona occludens-1 (ZO-1). Administration of XP + CS treatment in IBS-C rats significantly ameliorated the colonic histological parameters and exerted a positive effect on barrier integrity by restoring the expression of Occludin and zona occludens-1 (ZO-1). Our findings demonstrated that the efficacy of XP and CS in managing constipation in rats is due to the ability of these compounds to form a protective barrier fortifying intestinal integrity and gut functionality.
肠易激综合征是一种影响大肠的胃肠道疾病，其症状包括但不限于腹痛、腹胀和动力障碍。特别是，与便秘相关的肠易激综合征（IBS-C）的特征是大便干结、排便不畅和排便困难。尽管有几种药物可以改善肠道改变和便秘相关症状，但肠易激综合征的治疗仍然是一个挑战。众所周知，包括木葡聚糖、豌豆蛋白（XP）和奇亚籽粉（CS）在内的天然化合物在对抗多种胃肠道疾病方面具有有益作用。在此，我们旨在评估XP和CS联合治疗IBS-C大鼠模型中便秘相关改变的效果。通过胃内灌注2 mL冷水（0-4°C）14天诱导IBS-C，口服给予木葡聚糖、豌豆蛋白和奇亚籽（XP + CS）治疗7天。在第22天，收集结肠组织进行组织学分析。我们的结果表明，给予XP + CS可通过增加体重、食物和水摄入量来减轻便秘相关参数。在XP + CS治疗后，从第14天到第22天，粪便水分含量恢复到生理水平。IBS-C大鼠的结肠组织显示器官结构破坏并伴有水肿。紧密连接蛋白Occludin和闭合蛋白-1（ZO-1）表达的显著降低反映了结肠结构的丧失。在IBS-C大鼠中给予XP + CS治疗可显著改善结肠组织学参数，并通过恢复Occludin和闭合蛋白-1（ZO-1）的表达对屏障完整性产生积极影响。我们的研究结果表明，XP和CS在治疗大鼠便秘方面的功效归因于这些化合物形成保护屏障以加强肠道完整性和肠道功能的能力。
