College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, China.
Molecules. 2011 Dec 6;16(12):10104-22. doi: 10.3390/molecules161210104.
Total phenolic contents (TPC), total flavonoid contents (TFC), related antioxidative and antiradical capabilities of grape cane extracts from 11 varieties (five V. vinifera cultivars and six Chinese wild grapes) widely grown in China were evaluated. Antioxidant properties were determined as DPPH and ABTS radical-scavenging abilities, superoxide anion and hydroxyl radical and hydrogen peroxide scavenging assays, as well as reducing power. Phenolic profiles of the extracts were characterized by using high-performance liquid chromatography (HPLC) techniques. All extracts exhibited strong antioxidant and effective free radical inhibition activities (EC(50) values), which generally correlated negatively with TPC (r = -0.804 to -0.918) and TFC (r = -0.749 to -0.888). In comparison with gallic acid, Trolox and tert-butylhydroquinone (positive controls), most grape cane extracts showed more efficient scavenging effects toward different reactive oxygen species. HPLC analysis revealed the presence of (+)-catechin, (-)-epicatechin, and trans-resveratrol as major phenolic components in the extracts. These results suggest that grape cane extracts may serve as a potential source of natural antioxidant for food and pharmaceutical application.
测定了在中国广泛种植的 11 个品种（5 个 V. vinifera 品种和 6 个中国野生葡萄品种）的葡萄茎提取物的总酚含量（TPC）、总类黄酮含量（TFC）、相关抗氧化和抗自由基能力。抗氧化性能通过 DPPH 和 ABTS 自由基清除能力、超氧阴离子和羟自由基以及过氧化氢清除测定法以及还原能力来确定。采用高效液相色谱（HPLC）技术对提取物的酚类成分进行了表征。所有提取物均表现出较强的抗氧化和有效自由基抑制活性（EC（50）值），通常与 TPC（r = -0.804 至 -0.918）和 TFC（r = -0.749 至 -0.888）呈负相关。与没食子酸、Trolox 和叔丁基对苯二酚（阳性对照）相比，大多数葡萄茎提取物对不同活性氧具有更有效的清除作用。HPLC 分析表明，提取物中存在（+）-儿茶素、（-）-表儿茶素和反式白藜芦醇等主要酚类成分。这些结果表明，葡萄茎提取物可能是食品和制药应用中天然抗氧化剂的潜在来源。
