School of Ecology and Environmental Science, Yunnan University, Kunming 650504, China.
Biocontrol Engineering Research Center of Plant Disease and Pest, Biocontrol Engineering Research Center of Crop Disease and Pest, Kunming 650504, China.
Int J Mol Sci. 2022 Dec 2;23(23):15162. doi: 10.3390/ijms232315162.
Copper is an essential micronutrient for the maintenance of normal cell function but is toxic in excess. Dehydrins are group two late embryogenesis abundant proteins, which facilitate plant survival in harsh environmental conditions. Here, a YSK-type dehydrin, , was cloned from under copper toxicity and characterized using a heterologous expression system and in vitro or in vivo experiments and exhibited characteristics of intrinsic disorder during in vitro analyses. Heterologous expression of NtDHN17 enhanced the tolerance of to various metals, osmotic, and oxidative stress. NtDHN17 showed no Cu-binding properties in vivo or in vitro, indicating that metal ion binding is not universal among dehydrins. In vitro and in vivo experiments suggested that NtDHN17 behaved as a potent anti-aggregation agent providing strong protection to aggregated proteins induced by excess copper ions, an effect dependent on the K-segment but not on the Y- or S-segments. In summary, the protective role of NtDHN17 towards under conditions of copper toxicity may be related to anti-aggregation ability rather than its acting as an ion scavenger, which might be a valuable target for the genetic improvement of resistance to heavy metal stresses in plants.
铜是维持正常细胞功能所必需的微量元素，但过量则有毒。脱水素是第二类晚期胚胎丰富蛋白，有助于植物在恶劣环境条件下生存。在这里，从 中克隆出一种 YSK 型脱水素 ，并通过异源表达系统和体外或体内实验进行了表征，在体外分析中表现出固有无序的特征。NtDHN17 的异源表达增强了 对各种金属、渗透和氧化应激的耐受性。NtDHN17 在体内或体外均没有 Cu 结合特性，表明金属离子结合并非所有脱水素都具有普遍性。体外和体内实验表明，NtDHN17 作为一种有效的抗聚集剂，为过量铜离子诱导的聚集蛋白提供了强大的保护，这种作用依赖于 K 片段而不是 Y 或 S 片段。总之，NtDHN17 在铜毒性条件下对 的保护作用可能与其抗聚集能力有关，而与其作为离子清除剂的作用无关，这可能是植物耐重金属胁迫遗传改良的一个有价值的目标。
