Palomares-Rius Juan Emilio, Archidona-Yuste Antonio, Clavero-Camacho Ilenia, de la Fuente José A Carreira, Rey Ana, Viñegla Benjamín, Liébanas Gracia, Cantalapiedra-Navarrete Carolina, Castillo Pablo
Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avenida Menéndez Pidal s/n, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14004 Córdoba, Spain.
Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Centro Alameda del Obispo, 14004 Córdoba, Spain.
Plants (Basel). 2022 Dec 5;11(23):3385. doi: 10.3390/plants11233385.
species are obligate ectoparasitic nematodes on cultivated and wild herbaceous and woody plants occupying numerous soil categories. Several species may cause damage to several crops (. , , , and on carnation, lettuce, rose and walnut, respectively). This investigation proves and emphasizes the relevance of applying integrative taxonomy for the accurate detection of species in mountainous wild environments in the Malaga province, Southern Spain. This research analyzed 45 soil samples of maritimus pine and one of green heather in southern Spain and identified fourteen species, two of them are described herein as new species ( sp. nov., sp. nov.), six of them were first reports for Spain (, , , , sp. 2 SAS, and ), and six species (, , , , , and ) have been already reported in Spain. Accordingly, these data increase the biodiversity of pin nematodes in Spain comprising a total of 47 species (33.1% out of 142 total species of this genus). Phylogenetic analyses based on ribosomal and mitochondrial markers (D2-D3, ITS, and partial COI) resulted in a consistent position for the newly described species in this study ( sp. nov., sp. nov.). sp. nov. grouped in a separated subclade as unequivocal species from the -complex species (including and ), and sp. nov. clustered with , but clearly separate from this species. This study indicates that species diversity in natural environments may be higher than expected, and this study may help in accurate identifications.
该物种是寄生于栽培和野生草本及木本植物上的专性外寄生线虫，分布于多种土壤类型中。几种该物种可能会对几种作物造成损害（例如，分别对康乃馨、生菜、玫瑰和核桃造成损害）。本研究证明并强调了应用综合分类学在西班牙南部马拉加省山区野生环境中准确检测该物种的重要性。本研究分析了西班牙南部滨海松的45份土壤样本和一份绿色石南的土壤样本，鉴定出14种该物种，其中两种在此被描述为新物种（新种1、新种2），六种是西班牙首次报道（物种1、物种2、物种3、物种4、新种2 SAS、物种5），还有六种物种（物种6、物种7、物种8、物种9、物种10、物种11）已在西班牙被报道过。因此，这些数据增加了西班牙松材线虫的生物多样性至47种（占该属142种总数的33.1%）。基于核糖体和线粒体标记（D2-D3、ITS和部分COI）的系统发育分析得出了本研究中新描述的该物种（新种1、新种2）的一致位置。新种1聚为一个单独的亚分支，与类群复合体物种（包括物种12和物种13）明确区分，新种2与物种14聚类，但与该物种明显分开。本研究表明自然环境中的该物种多样性可能高于预期，且本研究有助于准确鉴定。
