Kim Myoungsub, Lee Dohui, Cho Hyun Suk, Chung Young-Soo, Park Hee Jin, Jung Ho Won
Department of Applied Bioscience, Dong-A University, Busan 49315, Korea.
Department of Molecular Genetics, Dong-A University, Busan 49315, Korea.
Plant Pathol J. 2022 Dec;38(6):603-615. doi: 10.5423/PPJ.OA.08.2022.0118. Epub 2022 Dec 1.
Soybean (Glycine max (L) Merr.) provides plant-derived proteins, soy vegetable oils, and various beneficial metabolites to humans and livestock. The importance of soybean is highly underlined, especially when carbon-negative sustainable agriculture is noticeable. However, many diseases by pests and pathogens threaten sustainable soybean production. Therefore, understanding molecular interaction between diverse cultivated varieties and pathogens is essential to developing disease-resistant soybean plants. Here, we established a pathosystem of the Korean domestic cultivar Kwangan against Pseudomonas syringae pv. syringae B728a. This bacterial strain caused apparent disease symptoms and grew well in trifoliate leaves of soybean plants. To examine the disease susceptibility of the cultivar, we analyzed transcriptional changes in soybean leaves on day 5 after P. syringae pv. syringae B728a infection. About 8,900 and 7,780 differentially expressed genes (DEGs) were identified in this study, and significant proportions of DEGs were engaged in various primary and secondary metabolisms. On the other hand, soybean orthologs to well-known plant immune-related genes, especially in plant hormone signal transduction, mitogen-activated protein kinase signaling, and plant-pathogen interaction, were mainly reduced in transcript levels at 5 days post inoculation. These findings present the feature of the compatible interaction between cultivar Kwangan and P. syringae pv. syringae B728a, as a hemibiotroph, at the late infection phase. Collectively, we propose that P. syringae pv. syringae B728a successfully inhibits plant immune response in susceptible plants and deregulates host metabolic processes for their colonization and proliferation, whereas host plants employ diverse metabolites to protect themselves against infection with the hemibiotrophic pathogen at the late infection phase.
大豆（Glycine max (L) Merr.）为人类和牲畜提供植物源蛋白质、大豆植物油以及各种有益代谢物。大豆的重要性得到了高度强调，尤其是在碳负可持续农业备受关注的当下。然而，许多病虫害威胁着大豆的可持续生产。因此，了解不同栽培品种与病原体之间的分子相互作用对于培育抗病大豆植株至关重要。在此，我们建立了韩国国内品种光安与丁香假单胞菌丁香致病变种B728a的病理系统。该菌株会引发明显的病害症状，并在大豆植株的三出复叶中良好生长。为检测该品种的病害易感性，我们分析了丁香假单胞菌丁香致病变种B728a感染后第5天大豆叶片中的转录变化。本研究鉴定出约8900个和7780个差异表达基因（DEG），且相当比例的DEG参与了各种初级和次级代谢。另一方面，与著名植物免疫相关基因同源的大豆基因，特别是在植物激素信号转导、丝裂原活化蛋白激酶信号传导和植物 - 病原体相互作用方面的基因，在接种后5天转录水平主要降低。这些发现呈现了光安品种与丁香假单胞菌丁香致病变种B728a在感染后期作为半活体营养菌的亲和性相互作用特征。总体而言，我们认为丁香假单胞菌丁香致病变种B728a成功抑制了感病植物的免疫反应，并失调宿主代谢过程以利于其定殖和增殖，而宿主植物在感染后期利用多种代谢物来保护自身免受半活体营养性病原体的感染。
