Department of Microbiology, National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.
Department of Genetics and Genetic Engineering, National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.
Folia Microbiol (Praha). 2023 Jun;68(3):403-414. doi: 10.1007/s12223-022-01016-w. Epub 2022 Dec 12.
Characterization of Cyanobacteria in lakes with different physicochemical properties provides insights into the diversity of this phylum and knowledge of their features that are relevant to biotechnology applications. Six Cyanobacterial isolates were recovered from freshwater Lake Nasser and saline Lake Qarun, Egypt. The isolates were identified based on both morphology and molecular markers, 16S rRNA, and RuBisCO cbbL genes. The isolates SN1, SN2, SN3, SN4, Q1, and Q2 showed homologies with Merismopedia, Oscillatoria, Limnothrix, Persinema, and Jacksonvillea, respectively. The cbbL sequences for isolates SN1, Q1, and Q2 represented the first records for candidates relating to the genera Merismopedia and Persinema, and Jacksonvillea, respectively. Biochemical contents, carbohydrates, proteins, lipids, pigments, and ash-free dry weight were measured for each isolate. Isolate SN2 had the highest content of allophycocyanin, 71 ± 4.8 mg/g DW, and phycoerythrin, 98 ± 6.7 mg/g DW, while the isolate SN4 had the highest composition of total protein, lipid, carotenoid, and chlorophyll a, recording 364.7 ± 6.4 mg/g DW, 67.6 ± 0.2 mg/g DW, 0.261 ± 0.01 mg/g DW, and 10 ± 0.6 mg/g DW, respectively. Isolate Q1 recorded the maximum amount of phycocyanin, 114 ± 20.7 mg/g DW among isolates. The isolate Q2 was observed to have the highest carbohydrate content, 274 ± 14.5 (mg/g DW), and ash-free dry weight, 891.8 ± 2.8 mg/g DW. Thus, the study indicated that the current isolates may represent promising resources for biotechnological applications.
从具有不同理化性质的湖泊中分离出的蓝藻进行特征描述，可以深入了解这一类群的多样性，并了解与生物技术应用相关的特征。从埃及淡水纳赛尔湖和盐水卡鲁恩湖共分离出 6 株蓝藻。基于形态学和分子标记（16S rRNA 和 RuBisCO cbbL 基因）对这些分离物进行了鉴定。分离物 SN1、SN2、SN3、SN4、Q1 和 Q2 分别与 Merismopedia、Oscillatoria、Limnothrix、Persinema 和 Jacksonvillea 同源。分离物 SN1、Q1 和 Q2 的 cbbL 序列分别代表 Merismopedia、Persinema 和 Jacksonvillea 相关属的首例记录。对每个分离物的碳水化合物、蛋白质、脂质、色素和无灰干重进行了测量。分离物 SN2 的藻蓝蛋白和藻红蛋白含量最高，分别为 71±4.8 mg/g DW 和 98±6.7 mg/g DW，而分离物 SN4 的总蛋白、脂质、类胡萝卜素和叶绿素 a 的含量最高，分别为 364.7±6.4 mg/g DW、67.6±0.2 mg/g DW、0.261±0.01 mg/g DW 和 10±0.6 mg/g DW。分离物 Q1 的藻蓝蛋白含量最高，为 114±20.7 mg/g DW。分离物 Q2 的碳水化合物含量最高，为 274±14.5（mg/g DW），无灰干重最高，为 891.8±2.8 mg/g DW。因此，本研究表明，目前的分离物可能是生物技术应用的有前途的资源。
