Liu Tiange, Wu Fan, Chen Kejing, Pan Bingna, Yin Xifeng, You Yilin, Song Zhixuan, Li Dan, Huang Dejian
National University of Singapore (Suzhou) Research Institute, Suzhou, China.
Suzhou Kosmode Biotechnology Company, Suzhou, China.
Front Nutr. 2022 Nov 24;9:1016020. doi: 10.3389/fnut.2022.1016020. eCollection 2022.
Sweet potato is widely consumed as a healthy and nutritive vegetable containing bioactive constituents for health promotion. This study investigated the beneficial impact of white-fleshed sweet potato extract (SPE) on high fat diet (HFD)-induced obese mice.
First, SPE, in which resin glycoside was found as the dominant constituent, was suggested as a potential anti-obesity agent, because 20-70% pancreatic lipase (PL) inhibition was measured with SPE by turbidity assay and pNPP assay. Hence, next, the effect of SPE on obese mice was detected by oral administration of HFD supplemented with 6% SPE on C57BL/6J mice for 9 weeks. Surprisingly, being the opposite of what was typically observed from a lipase inhibitor such as orlistat, the fecal fat content in SPE-fed obese mice was decreased ( < 0.01). Meanwhile, 6% SPE supplement indeed significantly ameliorated HFD-induced obesity in mice, including body weight gain, fat accumulation, adipocyte enlargement, insulin resistance, and hepatic steatosis ( < 0.05). The improved liver steatosis was found associated with a down-regulating action of SPE on nuclear factor kappa B activation in HFD-fed mice. The anti-obesity influence of SPE was also confirmed on the HepG2 cell model for non-alcoholic fatty liver disease (NAFLD).
These results indicate that SPE, as a dietary supplement, has the great potential for weight control and treating hepatic steatosis, possibly through a different action mechanism from that of orlistat.
红薯作为一种健康且营养丰富的蔬菜被广泛食用，它含有促进健康的生物活性成分。本研究调查了白肉红薯提取物（SPE）对高脂饮食（HFD）诱导的肥胖小鼠的有益影响。
首先，发现树脂糖苷为主要成分的SPE被认为是一种潜在的抗肥胖剂，因为通过比浊法和对硝基苯磷酸酯（pNPP）法测定，SPE对胰脂肪酶（PL）的抑制率为20 - 70%。因此，接下来，通过对C57BL/6J小鼠口服补充6% SPE的高脂饮食9周来检测SPE对肥胖小鼠的影响。令人惊讶的是，与通常从奥利司他等脂肪酶抑制剂观察到的情况相反，喂食SPE的肥胖小鼠粪便脂肪含量降低（<0.01）。同时,补充6% SPE确实显著改善了高脂饮食诱导的小鼠肥胖，包括体重增加、脂肪堆积、脂肪细胞增大、胰岛素抵抗和肝脂肪变性（<0.05）。发现肝脏脂肪变性的改善与SPE对高脂饮食喂养小鼠中核因子κB激活的下调作用有关。SPE对非酒精性脂肪性肝病（NAFLD）的HepG2细胞模型的抗肥胖影响也得到了证实。
这些结果表明，作为一种膳食补充剂，SPE在控制体重和治疗肝脂肪变性方面具有巨大潜力，其作用机制可能与奥利司他不同。
