Xiao Man, Chen Bocen, Niu Kun, Long Ziyu, Yang Fan, Xie Yiqiang
Key Laboratory of Biochemistry and Molecular Biology, Hainan Medical University, Haikou, Hainan, China.
College of Chinese Traditional Medicine, Hainan Medical University, Haikou, Hainan, China.
Front Pharmacol. 2022 Nov 24;13:1034515. doi: 10.3389/fphar.2022.1034515. eCollection 2022.
(AOF) is Traditional Chinese medicine and a dietary supplements for centuries, which posseses cardiotonic, neuroprotective, antioxidant, warming the kidney and nourish the spleen, these biological fuction is related to potential anti-aging properties. However, little is known about their effects on aging. This work aimed to investigate the effects of extracts of AOF on longevity and stress resistance in () and the mechanisms that underlie its effects. Wild-type (WT) strand of (N2)worms were cultured in growth medium with or without AOF. First, we examined the effects of AOF on lifespan, reproduction and healthspan assay, stress resistance and oxidative analysis, lipofuscin levels. Second, The levels of ROS and MDA, the antioxidant enzyme activities were examined to explore the underlying mechanism of AOF. Finally, the expression of the longevity-related genes were investigated to further understand the AOF's underlying mechanism. The lifespan of was prolonged by 23.44% after treatment with high-dose AOF (100 ug/ml). AOF alleviated aging-related declines in health and enhanced resistance to heat shock. Furthermore, AOF decreased reactive oxygen species and malondialdehyde, increased the activities of superoxide dismutase and catalase, and reduced accumulation of fat. AOF upregulated the expression of sod-3, gst-4, daf-16, and skn-1 but downregulated the expression of daf-2 and age-1 and accelerated the translocation of DAF-16 into the nucleus. The extended lifespan induced by AOF was reversed in and ) mutants, indicating that this gene is involved in AOF-regulated longevity. Our findings demonstrated that AOF extends lifespan and healthspan and enhances stress boosting the activity of the antioxidant enzyme and controlling the expression of genes associated with insulin/IGF signaling and SKN-1 pathways. As a result, this work suggested AOF as a possible candidate to reduce the signs of aging by activating and inhibiting target genes.
数世纪以来，（AOF）一直是一种中药和膳食补充剂，具有强心、神经保护、抗氧化、温肾健脾的作用，这些生物学功能与潜在的抗衰老特性有关。然而，人们对其衰老方面的影响知之甚少。这项研究旨在探究AOF提取物对（）寿命和抗应激能力的影响及其作用机制。野生型（WT）（N2）线虫在添加或不添加AOF的生长培养基中培养。首先，我们检测了AOF对寿命、繁殖和健康状况检测、抗应激能力和氧化分析、脂褐素水平的影响。其次，检测活性氧（ROS）和丙二醛水平以及抗氧化酶活性，以探究AOF的潜在作用机制。最后，研究与长寿相关基因的表达，以进一步了解AOF的潜在机制。高剂量AOF（100μg/ml）处理后，线虫的寿命延长了23.44%。AOF减轻了与衰老相关的健康衰退，并增强了对热休克的抵抗力。此外，AOF降低了活性氧和丙二醛水平，提高了超氧化物歧化酶和过氧化氢酶的活性，并减少了脂肪积累。AOF上调了sod-3、gst-4、daf-16和skn-1的表达，但下调了daf-2和age-1的表达，并加速了DAF-16向细胞核的转位。在（）和（）突变体中，AOF诱导的寿命延长被逆转，表明该基因参与了AOF调节的长寿过程。我们的研究结果表明，AOF可延长寿命和健康期，并增强应激能力，提高抗氧化酶活性，控制与胰岛素/胰岛素样生长因子信号通路和SKN-1通路相关基因的表达。因此，这项研究表明AOF可能是通过激活和抑制靶基因来减少衰老迹象的候选物质。
