Shu Qian, Xia Dong, Ma Yueyang, Zhang Yang, Luo Ting, Ma Jiaxin, Liu Fang, Yan Shuxing, Liu Daxiang
College of Biological & Pharmaceutical Sciences, China Three Gorges University, Yichang, China.
Hubei Provincial Engineering Research Center of Slope Habitat Construction Technique Using Cement-based Materials, China Three Gorges University, Key Laboratory of Mountain Hazards and Surface Processes Chinese, Yichang, China.
Front Plant Sci. 2022 Nov 23;13:1028553. doi: 10.3389/fpls.2022.1028553. eCollection 2022.
In order to solve the inhibition of alkaline environment on plants growth at the initial stage of Eco-restoration of vegetation concrete technology, introducing AMF into vegetation concrete substrate is an effective solution.
In this study, (GM), (GI) and a mixture of two AMF (MI) were used as exogenous inoculation agents. and were selected as host plants to explore the relationship between the physiological characteristics of plants and the content of substrate cement under exogenous inoculation of AMF.
The experiment showed that, for , the maximum mycorrhizal infection rates of inoculation with GM, MI were when the cement contents ranged 5-8% and that of GI inoculation was with the cement contents ranging 5-10%. Adversely, for , substrate cement content had little effect on the root system with the exogenous inoculation of AMF. Compared with CK, the effects of AMF inoculation on the physiological characteristics of the two plants were different. When the cement content was the highest (10% and 8% respectively), AMF could significantly increase(<0.05) the intercellular CO concentration (Ci) of . Moreover, for both plants, single inoculation was more effective than mixed inoculation. When the cement content was relatively low, the physiological characteristics of were promoted more obviously by the inoculation of GI. At higher cement content level, inoculation of GM had a better effect on the physiological characteristics of the two plants.
The results suggest that single inoculation of GM should be selected to promote the growth of and in higher alkaline environment.
为解决植被混凝土技术生态修复初期碱性环境对植物生长的抑制问题，将丛枝菌根真菌（AMF）引入植被混凝土基质是一种有效的解决方法。
本研究中，将摩西斗管囊霉（GM）、幼套球囊霉（GI）以及两种AMF的混合物（MI）用作外源接种剂。选用高羊茅和黑麦草作为宿主植物，以探究在AMF外源接种条件下植物生理特性与基质水泥含量之间的关系。
试验表明，对于高羊茅，接种GM、MI时菌根侵染率最高的水泥含量范围为5%-8%，接种GI时菌根侵染率最高的水泥含量范围为5%-10%。相反，对于黑麦草，AMF外源接种时基质水泥含量对根系影响较小。与对照相比，AMF接种对两种植物生理特性的影响不同。当水泥含量最高时（分别为10%和8%），AMF能显著增加（P<0.05）高羊茅的胞间CO₂浓度（Ci）。此外，对于两种植物，单接种比混合接种更有效。当水泥含量相对较低时，接种GI对黑麦草生理特性的促进作用更明显。在水泥含量较高时，接种GM对两种植物生理特性的影响更好。
结果表明，应选择单接种GM来促进高羊茅和黑麦草在高碱性环境中的生长。
