Bolik-Coulon Nicolas, Sever Alexander I M, Harkness Robert W, Aramini James M, Toyama Yuki, Hansen D Flemming, Kay Lewis E
Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada; Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada; Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada.
Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada.
J Magn Reson. 2023 Jan;346:107326. doi: 10.1016/j.jmr.2022.107326. Epub 2022 Nov 9.
The HMQC pulse sequence and variants thereof have been exploited in studies of high molecular weight protein complexes, taking advantage of the fact that fast and slow relaxing magnetization components are sequestered along two distinct magnetization transfer pathways. Despite the simplicity of the HMQC scheme an even shorter version can be designed, based on elimination of the terminal refocusing period, as a further means of increasing signal. Here we present such an experiment, and show that significant sensitivity gains, in some cases by factors of two or more, are realized in studies of proteins varying in molecular masses from 100 kDa to 1 MDa.
HMQC脉冲序列及其变体已被用于高分子量蛋白质复合物的研究中，这是利用了快速和慢速弛豫磁化分量沿着两条不同的磁化转移途径被隔离这一事实。尽管HMQC方案很简单，但基于消除末端重聚焦期，仍可设计出更短的版本，作为进一步提高信号的一种方法。在此，我们展示了这样一个实验，并表明在分子量从100 kDa到1 MDa不等的蛋白质研究中，实现了显著的灵敏度提高，在某些情况下提高了两倍或更多。
