Ma Xianzong, Lu Xiaojuan, Zhang Wenyu, Yang Lang, Wang Dezhi, Xu Junfeng, Jia Yan, Wang Xin, Xie Hui, Li Shu, Zhang Mingjie, He Yuqi, Jin Peng, Sheng Jianqiu
Medical School of Chinese PLA, Beijing, 100853, China.
Department of Gastroenterology, The Seventh Medical Center of Chinese PLA General Hospital, No.5 Nanmencang, Beijing, 100700, China.
Gut Pathog. 2022 Dec 14;14(1):46. doi: 10.1186/s13099-022-00521-0.
Emerging evidence suggests that gut microbiota plays a predominant role in Crohn's disease (CD). However, the microbiome alterations in the early stage of CD patients still remain unclear. The present study aimed to identify dysbacteriosis in patients with early CD and explore specific gut bacteria related to the progression of CD.
This study was nested within a longitudinal prospective Chinese CD cohort, and it included 18 early CD patients, 22 advanced CD patients and 30 healthy controls. The microbiota communities were investigated using high-throughput Illumina HiSeq sequencing targeting the V3-V4 region of 16S ribosomal DNA (rDNA) gene. The relationship between the gut microbiota and clinical characteristics of CD was analyzed.
Differential microbiota compositions were observed in CD samples (including early and advanced CD samples) and healthy controls samples. Notably, Lachnospiracea_incertae_sedis and Parabacteroides were enriched in the early CD patients, Escherichia/Shigella, Enterococcus and Proteus were enriched in the advanced CD patients, and Roseburia, Gemmiger, Coprococcus, Ruminococcus 2, Butyricicoccus, Dorea, Fusicatenibacter, Anaerostipes, Clostridium IV were enriched in the healthy controls [LDA score (log10) > 2]. Furthermore, Kruskal-Wallis Rank sum test results showed that Blautia, Clostridium IV, Coprococcus, Dorea, Fusicatenibacter continued to significantly decrease in early and advanced CD patients, and Escherichia/Shigella and Proteus continued to significantly increase compared with healthy controls (P < 0.05). The PICRUSt analysis identified 16 remarkably different metabolic pathways [LDA score (log10) > 2]. Some genera were significantly correlated with various clinical parameters, such as fecal calprotectin, erythrocyte sedimentation rate, C-reactive protein, gland reduce, goblet cells decreased, clinical symptoms (P < 0.05).
Dysbacteriosis occurs in the early stage of CD and is associated with the progression of CD. This data provides a foundation that furthers the understanding of the role of gut microbiota in CD's pathogenesis.
新出现的证据表明，肠道微生物群在克罗恩病（CD）中起主要作用。然而，CD患者早期的微生物组改变仍不清楚。本研究旨在确定早期CD患者的菌群失调情况，并探索与CD进展相关的特定肠道细菌。
本研究嵌套于一个纵向前瞻性中国CD队列中，纳入了18例早期CD患者、22例晚期CD患者和30例健康对照。使用靶向16S核糖体DNA（rDNA）基因V3-V4区域的高通量Illumina HiSeq测序来研究微生物群落。分析肠道微生物群与CD临床特征之间的关系。
在CD样本（包括早期和晚期CD样本）和健康对照样本中观察到微生物群组成存在差异。值得注意的是，早期CD患者中未分类的毛螺菌科和副拟杆菌属富集，晚期CD患者中埃希氏菌属/志贺氏菌属、肠球菌属和变形杆菌属富集，健康对照中罗氏菌属、Gemmiger菌属、粪球菌属、瘤胃球菌2属、丁酸球菌属、多雷亚菌属、梭菌属IV、厌氧棒状菌属、厌氧芽孢梭菌属富集[线性判别分析（LDA）分数（log10）>2]。此外，Kruskal-Wallis秩和检验结果显示，与健康对照相比，早期和晚期CD患者中Blautia菌属、梭菌属IV、粪球菌属、多雷亚菌属、梭菌属IV持续显著减少，而埃希氏菌属/志贺氏菌属和变形杆菌属持续显著增加（P<0.05）。PICRUSt分析确定了16条显著不同的代谢途径[LDA分数（log10）>2]。一些菌属与各种临床参数显著相关，如粪便钙卫蛋白、红细胞沉降率、C反应蛋白、腺体减少、杯状细胞减少、临床症状（P<0.05）。
CD早期出现菌群失调，且与CD的进展相关。该数据为进一步理解肠道微生物群在CD发病机制中的作用奠定了基础。
