Peng Jianzhong, Wang Tao, Yue Chao, Luo Xianyan, Xiao Peng
Department of Dermatologic Surgery, Hangzhou Third People's Hospital, Hangzhou, Zhejiang, China.
Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Front Oncol. 2022 Nov 29;12:1004511. doi: 10.3389/fonc.2022.1004511. eCollection 2022.
Cutaneous melanoma is the deadliest type of skin cancer, and its highly aggressive and metastatic nature leads to an extremely poor prognosis. Necrotizing apoptosis, a specific form of programmed cell death, has been extensively studied in recent years. In this study, we analyzed the relationship between necroptosis-related functional genes and cutaneous melanoma in order to identify the biomarkers associated with the prognosis and progression of cutaneous melanoma. Cutaneous melanoma samples were classified into three subgroups on the basis of a necroptosis gene set. These subgroups were subjected to a prognostic survival analysis, and the greatest differences were observed between subgroups C1 and C3. Between these subgroups, 28 necrotizing apoptosis-related genes were significantly differently expressed. Among these, 16 necrotizing apoptosis-related genes were associated with cutaneous melanoma prognosis. Downscaling analysis and prognostic modeling using the least absolute shrinkage and selection operator analysis yielded nine pivotal genes and revealed phosphoglycerate translocase 5 (PGAM5) as the key gene. Then, qRT-PCR was used to verify the expression level of PGAM5. The results showed that PGAM5 was highly expressed in cutaneous melanoma tissues. In this study, a bioinformatics approach was used to identify PGAM5, a biomarker whose high expression is associated with the poor prognosis of cutaneous melanoma.
皮肤黑色素瘤是最致命的皮肤癌类型，其高度侵袭性和转移性导致预后极差。坏死性凋亡作为程序性细胞死亡的一种特殊形式，近年来已得到广泛研究。在本研究中，我们分析了坏死性凋亡相关功能基因与皮肤黑色素瘤之间的关系，以确定与皮肤黑色素瘤预后和进展相关的生物标志物。基于坏死性凋亡基因集，将皮肤黑色素瘤样本分为三个亚组。对这些亚组进行预后生存分析，发现C1和C3亚组之间差异最大。在这些亚组之间，28个坏死性凋亡相关基因的表达存在显著差异。其中，16个坏死性凋亡相关基因与皮肤黑色素瘤预后相关。使用最小绝对收缩和选择算子分析进行降维分析和预后建模，得到9个关键基因，并揭示磷酸甘油转运酶5（PGAM5）为关键基因。然后，采用qRT-PCR验证PGAM5的表达水平。结果显示，PGAM5在皮肤黑色素瘤组织中高表达。在本研究中，采用生物信息学方法鉴定出PGAM5，这是一种高表达与皮肤黑色素瘤预后不良相关的生物标志物。
