Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health. Hangzhou 310052, PR China.
School of Public Health, Zhejiang University School of Medicine, Hangzhou 310052, PR China.
J Clin Virol. 2023 Jan;158:105354. doi: 10.1016/j.jcv.2022.105354. Epub 2022 Dec 10.
Respiratory syncytial virus (RSV) is one of the main pathogens that causes acute lower respiratory tract infection (ARTI) in infants. During the Coronavirus Disease 2019 (COVID-19) pandemic, although strict interventions have been implemented, RSV infection has not decreased.
To study the epidemiological and genetic characteristics of RSV circulating in Hangzhou after the peak of COVID-19.
A total of 1225 nasopharyngeal swabs were collected from outpatients with ARTIs from July 2021 to January 2022 in The Children's Hospital, Zhejiang University School of Medicine.
A total of 267 (21.79%) of the 1225 samples were RSV positive. There was no gender bias. However, an obvious age preference for infection was observed, and children aged 3-6 years were more susceptible, which was very different from previous RSV pandemic seasons. Phylogenetic analysis of 115 sequenced RSV isolates showed that all the RSV-A viruses belong to the ON1 subtype, which could be clustered into three clusters. While all the RSV-B viruses belong to BA9. Further analysis of the mutations highlights the fixation of ten mutations, which should be given extra attention regarding their biological properties.
The incidence of RSV infection in preschool children reported in this study is high. Phylogenetic analysis showed that the subtype A ON1 genotype was the dominant strain in Hangzhou from July 2021 to January 2022.
呼吸道合胞病毒（RSV）是导致婴幼儿急性下呼吸道感染（ARTI）的主要病原体之一。在 2019 年冠状病毒病（COVID-19）大流行期间，尽管采取了严格的干预措施，但 RSV 感染并未减少。
研究 COVID-19 高峰后杭州流行的 RSV 的流行病学和遗传特征。
2021 年 7 月至 2022 年 1 月，从浙江大学医学院附属儿童医院门诊收治的急性呼吸道感染患儿中采集 1225 例鼻咽拭子。
1225 份标本中，267 份（21.79%）为 RSV 阳性。男女之间无性别差异。然而，感染存在明显的年龄偏好，3-6 岁儿童易感染，与以往 RSV 大流行季节明显不同。115 株 RSV 分离株的系统进化分析表明，所有 RSV-A 病毒均属于 ON1 亚型，可分为 3 个簇。而所有 RSV-B 病毒均属于 BA9。进一步分析突变，突出了 10 个突变的固定，应特别注意其生物学特性。
本研究报道的学龄前儿童 RSV 感染发生率较高。系统进化分析显示，2021 年 7 月至 2022 年 1 月，杭州流行的 ON1 基因型 A 是优势株。
