超声处理杀死的后生元对小鼠实验性三阴性乳腺肿瘤的抑制作用：一项初步研究。
Inhibitory effects of postbiotic consisting sonication-killed on experimental triple negative breast neoplasm in mice: a preliminary study.
作者信息
Nazari Farzaneh, Jafari Parvaneh, Nomanpour Bizhan, Varmira Kambiz, Raissi Farshid
机构信息
Department of Microbiology, Faculty of Sciences, Islamic Azad University, Arak Branch, Arak, Iran.
Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
出版信息
Iran J Microbiol. 2022 Oct;14(5):689-697. doi: 10.18502/ijm.v14i5.10964.
BACKGROUND AND OBJECTIVES
Breast cancer is the second leading cause of death and one of the most common malignancies among women in the world. The aim of this study was to investigate the preventive effects of postbiotic consisting of sonicated cells on triple negative breast cancer.
MATERIALS AND METHODS
Thirty-six female BALB/c mice aged 5-7 weeks were randomly divided into 3 groups (n=12): Ctrl-, healthy mice; Ctrl+, mice with breast cancer with no treatment; and Postbiotic, mice with orally gavage postbiotic before and after 4T1 cell line transplantation. Cancer progress and the effects of postbiotic were assayed by histological, immunohistochemical and gene expression quantification.
RESULTS
The histological results showed that administration postbiotic consisting of significantly decreased carcinogenesis in terms of tumor incidence, multiplicity and volume. The tumor progress was suppressed by oral intake of as showed by p53 and Ki-67 expression. Furthermore, Oral intake of postbiotic resulted in extended survival of mice and inhibited sever weigh loss.
CONCLUSION
Pretreatment with sonication killed as a postbiotic, inhibited breast cancer progress and malignancy.
背景与目的
乳腺癌是全球女性中第二大死因，也是最常见的恶性肿瘤之一。本研究旨在探讨由超声处理过的细胞组成的后生元对三阴性乳腺癌的预防作用。
材料与方法
将36只5至7周龄的雌性BALB/c小鼠随机分为3组（n = 12）：对照组-，健康小鼠；对照组+，未接受治疗的乳腺癌小鼠；后生元组，在移植4T1细胞系前后经口灌胃后生元的小鼠。通过组织学、免疫组织化学和基因表达定量分析癌症进展和后生元的作用。
结果
组织学结果表明，由[具体内容缺失]组成的后生元给药在肿瘤发生率、多发性和体积方面显著降低了致癌作用。如p53和Ki-67表达所示，口服[具体内容缺失]可抑制肿瘤进展。此外，口服后生元可延长小鼠生存期并抑制体重严重减轻。
结论
用超声处理杀死的[具体内容缺失]作为后生元进行预处理，可抑制乳腺癌进展和恶性程度。
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