Öğüt Neslihan Demirel, Hasçelik Gülşen, Atakan Nilgün
Hacettepe University, Department of Dermatology and Venereology, Ankara, Turkey.
Hacettepe University, Department of Medical Microbiology, Ankara, Turkey.
Dermatol Pract Concept. 2022 Oct 1;12(4):e2022191. doi: 10.5826/dpc.1204a191. eCollection 2022 Nov.
Hidradenitis suppurativa (HS) is a chronic and systemic inflammatory disease that extends beyond the skin. The role of gut microbiome (GM) alterations in the pathogenesis of inflammatory and autoimmune disorders is remarkable.
Based on the hypothesis that dysbiosis in the GM may trigger systemic inflammation in the pathogenesis of HS, this study aimed to investigate whether the GM is altered in HS patients compared with healthy subjects.
In the present case-control study, fecal samples from 15 patients with HS and 15 age- and sex-matched healthy individuals were collected and analyzed using 16S rRNA-based metagenomic analysis, New Generation Sequencing (NGS). The V3 and V4-hypervariable regions of the bacterial 16S rDNA gene were amplified from all samples and sequenced by the Illumina MiSeq platform. Bioinformatics analyses were performed in QIIME2.
Shannon alpha diversity index showed significantly reduced diversity in HS patients (P = 0.048). Bray-Curtis Dissimilarity and Jaccard Distance revealed that the gut microbial composition of HS patients was significantly distinctive from that of controls (P = 0.01 and P = 0.007, respectively). The relative abundance of unclassified Clostridiales, unclassified Firmicutes, and Fusicatenibacter in HS was significantly lower than that in controls (P = 0.005, P = 0.029, and P = 0.046, respectively).
This study indicated that significant alterations in the GM of HS patients could play a critical role in the pathogenesis of HS and might be a trigger for systemic inflammation. Increased understanding of the pathogenesis of HS will shed light on the new potential therapeutic targets and novel treatment options.
化脓性汗腺炎（HS）是一种慢性全身性炎症性疾病，其影响范围超出皮肤。肠道微生物群（GM）改变在炎症性和自身免疫性疾病发病机制中的作用显著。
基于GM生态失调可能在HS发病机制中引发全身炎症这一假设，本研究旨在调查与健康受试者相比，HS患者的GM是否发生改变。
在本病例对照研究中，收集了15例HS患者和15例年龄及性别匹配的健康个体的粪便样本，并使用基于16S rRNA的宏基因组分析、新一代测序（NGS）进行分析。从所有样本中扩增细菌16S rDNA基因的V3和V4高变区，并通过Illumina MiSeq平台进行测序。在QIIME2中进行生物信息学分析。
香农α多样性指数显示HS患者的多样性显著降低（P = 0.048）。布雷-柯蒂斯差异和杰卡德距离显示，HS患者的肠道微生物组成与对照组有显著差异（分别为P = 0.01和P = 0.007）。HS中未分类的梭菌目、未分类的厚壁菌门和梭菌属的相对丰度显著低于对照组（分别为P = 0.005、P = 0.029和P = 0.046）。
本研究表明，HS患者GM的显著改变可能在HS发病机制中起关键作用，可能是全身炎症的触发因素。对HS发病机制的深入了解将为新的潜在治疗靶点和新的治疗选择提供线索。
