刺突蛋白中 Q677H 突变的 SARS-CoV-2 变异株的分离与鉴定。
Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein.
机构信息
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
Center for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH, USA.
出版信息
Arch Virol. 2022 Dec 20;168(1):5. doi: 10.1007/s00705-022-05621-5.
We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020-January 2021.
我们从俄亥俄州哥伦布市采集的阳性临床样本中分离出 20 株 SARS-CoV-2 毒株，并研究了其中一对分离株的复制情况：一株 20G 谱系株和该谱系株的一个变体，其在刺突蛋白中携带 Q677H 突变和另外六个氨基酸突变。在 Vero-E6 细胞中，OSU.20G 变体的复制达到了比 20G 原始株更高的感染滴度峰值，但当两种毒株在 Calu-3 细胞中生长时，滴度相似。这些结果表明，与 20G 原始株相比，OSU.20G 变体的复制适应性增强。这可能促成了它在 2020 年 12 月至 2021 年 1 月的出现。
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