Huang Xintao, Xu Xinjuan, Wang Ce, Wang Yi, Yang Yajun, Yao Tianle, Bai Rui, Pei Xile, Bai Feirong, Li Panpan
Department of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
Department of Neurosurgery, Shanxi Cardiovascular Hospital, Taiyuan, Shanxi, China.
Front Neurosci. 2023 Apr 18;17:1145307. doi: 10.3389/fnins.2023.1145307. eCollection 2023.
Traumatic brain injury (TBI) is considered the most common traumatic neurological disease, is associated with high mortality and long-term complications, and is a global public health issue. However, there has been little progress in serum markers for TBI research. Therefore, there is an urgent need for biomarkers that can sufficiently function in TBI diagnosis and evaluation.
Exosomal microRNA (ExomiR), a stable circulating marker in the serum, has aroused widespread interest among researchers. To explore the level of serum ExomiR after TBI, we quantified ExomiR expression levels in serum exosomes extracted from patients with TBI using next-generation sequencing (NGS) and explored potential biomarkers using bioinformatics screening.
Compared with the control group, there were 245 ExomiR (136 up-regulated and 109 down-regulated) in the serum of the TBI group that changed significantly. We observed serum ExomiRs expression profiles associated with neurovascular remodeling, the integrity of the blood-brain barrier, neuroinflammation, and a cascade of secondary injury, including eight up-regulated ExomiRs (ExomiR-124-3p, ExomiR-137-3p, ExomiR-9-3p, ExomiR-133a-5p, ExomiR-204-3p, ExomiR-519a-5p, ExomiR-4732-5p, and ExomiR-206) and 2 down-regulated ExomiR (ExomiR-21-3p and ExomiR-199a-5).
The results revealed that serum ExomiRs might become a new research direction and breakthrough for the diagnosis and pathophysiological treatment of patients with TBI.
创伤性脑损伤（TBI）被认为是最常见的创伤性神经系统疾病，与高死亡率和长期并发症相关，是一个全球公共卫生问题。然而，TBI研究的血清标志物进展甚微。因此，迫切需要能够在TBI诊断和评估中充分发挥作用的生物标志物。
外泌体微小RNA（ExomiR）是血清中一种稳定的循环标志物，已引起研究人员的广泛关注。为了探究TBI后血清ExomiR的水平，我们使用下一代测序（NGS）对从TBI患者提取的血清外泌体中的ExomiR表达水平进行定量，并通过生物信息学筛选探索潜在的生物标志物。
与对照组相比，TBI组血清中有245种ExomiR（136种上调和109种下调）发生了显著变化。我们观察到血清ExomiRs表达谱与神经血管重塑、血脑屏障完整性、神经炎症以及一系列继发性损伤相关，包括8种上调的ExomiR（ExomiR-124-3p、ExomiR-137-3p、ExomiR-9-3p、ExomiR-133a-5p、ExomiR-204-3p、ExomiR-519a-5p、ExomiR-4732-5p和ExomiR-206）和2种下调的ExomiR（ExomiR-21-3p和ExomiR-199a-5）。
结果表明，血清ExomiRs可能成为TBI患者诊断和病理生理治疗的新研究方向和突破点。
