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电化学生物传感器用于检测 microRNA 作为癌症生物标志物：优缺点。

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons.

机构信息

Laboratory of Process Engineering & Environment, Faculty of Sciences and Techniques, Hassan II, University of Casablanca, B.P.146, Mohammedia 28806, Morocco.

Université Paris-Saclay, CNRS, Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO), Equipe de Chimie Biorganique et Bioinorganique (ECBB), Bât 420, 2 Rue du Doyen Georges Poitou, 91400 Orsay, France.

出版信息

Biosensors (Basel). 2020 Nov 20;10(11):186. doi: 10.3390/bios10110186.

DOI:10.3390/bios10110186
PMID:33233700
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7699780/
Abstract

Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

摘要

癌症是世界上第二大致命疾病，早期诊断对于成功治疗至关重要。因此，有必要开发快速、敏感、简单且廉价的癌症生物标志物分析工具。MicroRNA（miRNA）是一种 RNA 癌症生物标志物，其在体液中的表达水平与癌症密切相关。已经开发出了各种涉及 miRNA 检测的用于癌症诊断的生物传感器。本综述提供了 2015 年至 2020 年间电化学生物传感器用于 miRNA 癌症标志物检测的最新进展的全面概述。综述重点介绍了基于电化学信号的直接 miRNA 检测方法。它包括具有不同设计的 RedOx 标记探针、RedOx DNA 嵌入剂、用于产生信号响应的各种 RedOx 催化剂，以及最终的自由 RedOx 指示剂。此外，还强调了这些方法的优缺点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71a0/7699780/bf08d683ff84/biosensors-10-00186-g017.jpg
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