Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, Delhi, India.
Amity Centre for Nanomedicine, Amity University, Noida 201301, Uttar Pradesh, India.
Biosensors (Basel). 2022 Dec 7;12(12):1144. doi: 10.3390/bios12121144.
Anisotropic gold nanostructures have fascinated with their exceptional electronic properties, henceforth exploited for the fabrication of electrochemical sensors. However, their synthesis approaches are tedious and often require a growth template. Modern lifestyle has caused an upsurge in the risk of heart attack and requires urgent medical attention. Cardiac troponin I can serve as a biomarker in identification of suspected myocardial infection (heart attack). Hence the present work demonstrates the fabrication of a sensing platform developed by assimilating anisotropic gold nanoclusters (AuNCs) with anti cTnI antibody (acTnI) for the detection of cardiac troponin I (cTnI). The uniqueness and ease of synthesis by a template-free approach provides an extra edge for the fabrication of AuNC coated electrodes. The template-free growth of anisotropic AuNCs onto the indium tin oxide (ITO) glass substrates offers high sensitivity (2.2 × 10 A ng mL cm) to the developed sensor. The immunosensor was validated by spiking different concentrations of cTnI in artificial serum with negligible interference under optimized conditions. The sensor shows a wide range of detection from 0.06-100 ng/mL with an ultralow detection limit. Thus, it suggests that the template-free immunosensor can potentially be used to screen the traces of cTnI present in blood serum samples, and the AuNCs based platform holds great promise as a transduction matrix, hence it can be exploited for broader sensing applications.
各向异性金纳米结构以其独特的电子特性而备受关注，因此被用于电化学传感器的制造。然而，它们的合成方法繁琐，通常需要生长模板。现代生活方式导致心脏病发作的风险急剧上升，需要紧急医疗关注。心肌肌钙蛋白 I 可以作为疑似心肌感染（心脏病发作）的生物标志物。因此，本工作展示了一种通过整合各向异性金纳米团簇（AuNCs）与抗 cTnI 抗体（acTnI）来制备感测平台的方法，用于检测心肌肌钙蛋白 I（cTnI）。无模板方法的独特性和易于合成为 AuNC 涂层电极的制备提供了额外的优势。各向异性 AuNCs 在铟锡氧化物（ITO）玻璃基底上的无模板生长为开发的传感器提供了高灵敏度（2.2 × 10 A ng mL cm）。在优化条件下，该免疫传感器通过在人工血清中掺入不同浓度的 cTnI 进行验证，结果表明几乎没有干扰。该传感器的检测范围从 0.06-100 ng/mL 宽，检测限超低。因此，该无模板免疫传感器有望用于筛选血清样本中 cTnI 的痕量，基于 AuNC 的平台作为一种转导基质具有很大的应用潜力，因此可以用于更广泛的传感应用。
