CSIR-Central Scientific Instruments Organization, Sector 30-C, Chandigarh 160030, India; CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110007, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
CSIR-Central Scientific Instruments Organization, Sector 30-C, Chandigarh 160030, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India.
Biosens Bioelectron. 2016 May 15;79:495-9. doi: 10.1016/j.bios.2015.12.083. Epub 2015 Dec 25.
Cardiac immunosensor for early detection of heart attack (myocardial infarction) was developed using amine functionalized graphene quantum dots (afGQDs) conjugated with antibody anti-cardiac Troponin I (anti-cTnI) to detect cardiac marker antigen Troponin I (cTnI) in blood based on fluorescence resonance energy transfer (FRET) between conjugate and graphene (quencher) only in 10 min. The anti-cTnI was covalently conjugated to afGQDs through carbodiimide coupling reaction. The conjugate was characterized by zeta potential UV-vis spectroscopy and field emission scanning electron microscopy (FESEM). The sensing performance of the sensor was studied with respect to changes in the photon count and photoluminescence of GQDs based on interaction of target cTnI with its specific anti-cTnI antibody. The sensor is highly specific and shows negligible response to non-specific antigens. The sensor displayed a linear response to cTnI from 0.001 to 1000 ng mL(-1) with a limit of detection of 0.192 pg mL(-1).
基于缀合物与石墨烯（猝灭剂）之间的荧光共振能量转移（FRET），使用胺功能化石墨烯量子点（afGQDs）与抗心肌肌钙蛋白 I（抗-cTnI）抗体偶联，开发了用于早期检测心脏病发作（心肌梗死）的心脏免疫传感器，以检测血液中的心脏标志物抗原肌钙蛋白 I（cTnI）。通过碳二亚胺偶联反应将抗-cTnI 共价偶联到 afGQDs 上。通过 ζ 电位紫外可见光谱和场发射扫描电子显微镜（FESEM）对偶联物进行了表征。基于目标 cTnI 与其特异性抗-cTnI 抗体的相互作用，研究了传感器对基于 GQDs 的光子计数和光致发光变化的传感性能。该传感器具有高度特异性，对非特异性抗原几乎没有响应。该传感器对 cTnI 的线性响应范围为 0.001 至 1000 ng mL(-1)，检测限为 0.192 pg mL(-1)。
