Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Biosensors (Basel). 2022 Dec 7;12(12):1145. doi: 10.3390/bios12121145.
The demand for glucose uptake and the accompanying enhanced glycolytic energy metabolism is one of the most important features of cancer cells. Unlike the aerobic metabolic pathway in normal cells, the large amount of pyruvate produced by the dramatic increase of glycolysis in cancer cells needs to be converted to lactate in the cytoplasm, which cannot be done without a large amount of lactate dehydrogenase (LDH). This explains why elevated serum LDH concentrations are usually seen in cancer patient populations. LDH not only correlates with clinical prognostic survival indicators, but also guides subsequent drug therapy. Besides their role in cancers, LDH is also a biomarker for malaria and other diseases. Therefore, it is urgent to develop methods for sensitive and convenient LDH detection. Here, this review systematically summarizes the clinical impact of lactate dehydrogenase detection and principles for LDH detection. The advantages as well as limitations of different detection methods and the future trends for LDH detection were also discussed.
对葡萄糖摄取的需求以及随之而来的增强的糖酵解能量代谢是癌细胞的最重要特征之一。与正常细胞中的有氧代谢途径不同，癌细胞中糖酵解急剧增加所产生的大量丙酮酸需要在细胞质中转化为乳酸，而这没有大量的乳酸脱氢酶 (LDH) 是无法完成的。这就解释了为什么癌症患者群体中通常会出现血清 LDH 浓度升高的情况。LDH 不仅与临床预后生存指标相关，还指导后续的药物治疗。除了在癌症中的作用外，LDH 还是疟疾和其他疾病的生物标志物。因此，迫切需要开发灵敏便捷的 LDH 检测方法。在这里，本文系统地总结了乳酸脱氢酶检测的临床影响以及 LDH 检测的原理。还讨论了不同检测方法的优缺点以及 LDH 检测的未来趋势。
Zotero 插件
