Department of Clinical Biochemistry, Faculty of Medicine, King Abdulaziz University, Jeddah 21551, Saudi Arabia.
Saudi Diabetes Research Group, King Fahd Medical Research Centre, King Abdulaziz University, Jeddah 3270, Saudi Arabia.
Genes (Basel). 2022 Dec 2;13(12):2277. doi: 10.3390/genes13122277.
Studies suggest that ATP-binding cassette transporter A1 ( C69T) polymorphism is associated with a decreased incidence of type 2 diabetes mellitus (T2DM) and that there is an association between C69T polymorphism and the risk of dyslipidemia in diabetic individuals. However, other studies contradict these suggestions. Therefore, we aimed to investigate the prevalence of C69T (rs1800977) gene polymorphism in a representative sample of the Saudi population not previously diagnosed with diabetes and its possible association with dyslipidemia and dysglycemia. A cross-sectional design was used to recruit nondiabetic adults of both genders from the Saudi population in Jeddah by employing a stratified, two-stage cluster sampling method. A total of 650 people (337 men and 313 women) were recruited. Demographic, dietary, and lifestyle variables, as well as medical history and family history of chronic diseases, were collected using a predesigned questionnaire. Fasting blood samples were taken for the determination of fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), and lipids profile, which were followed by a 1-h oral glucose tolerance test (OGTT). Real-time PCR technology was used to determine the C69T gene SNP (rs1800977). The T allele of C69T (rs1800977) was very frequent (TT in 44.9% and CT in 43.7%). There was a trend toward significance for a higher dysglycemia percentage in people with CT and TT genotypes (25.7%, and 23.3%, respectively) compared with CC genotypes (16.2%). In addition, FPG and 1-h plasma glucose were significantly higher in people with both TT and CT genotypes compared to CC genotypes. However, T allele was not associated with any dysregulation of lipid parameters.
研究表明，三磷酸腺苷结合盒转运体 A1（C69T）多态性与 2 型糖尿病（T2DM）的发病率降低有关，并且 C69T 多态性与糖尿病个体的血脂异常风险之间存在关联。然而，其他研究对这些建议提出了质疑。因此，我们旨在调查先前未被诊断为糖尿病的沙特人群中 C69T（rs1800977）基因多态性的流行情况及其与血脂异常和糖代谢异常的可能关联。采用分层两阶段聚类抽样方法，在吉达的沙特人群中招募了未患糖尿病的男女两性的非糖尿病成年人，采用横断面设计。共招募了 650 人（337 名男性和 313 名女性）。使用预先设计的问卷收集了人口统计学、饮食和生活方式变量以及慢性病的病史和家族史。抽取空腹血样以测定空腹血糖（FPG）、糖化血红蛋白（HbA1c）和血脂谱，随后进行 1 小时口服葡萄糖耐量试验（OGTT）。实时 PCR 技术用于确定 C69T 基因 SNP（rs1800977）。C69T（rs1800977）的 T 等位基因非常常见（TT 为 44.9%，CT 为 43.7%）。与 CC 基因型相比，CT 和 TT 基因型的人（分别为 25.7%和 23.3%）出现糖代谢异常的百分比呈上升趋势，但无统计学意义。此外，与 CC 基因型相比，TT 和 CT 基因型的人 FPG 和 1 小时血浆葡萄糖均显著升高。然而，T 等位基因与任何血脂参数失调均无关。
