Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland.
Genes (Basel). 2022 Dec 19;13(12):2409. doi: 10.3390/genes13122409.
Identification of new, effective disease resistance genes is a very important aspect of plant breeding. Also important is the precise location of individual loci and tagging them with DNA markers for marker assisted selection. The aim of the present study was identification of the molecular markers linked with , a new effective resistance gene to powdery mildew, and their location in the oat genome. The analysis was performed on 167 F individuals from a hybrid of Fuchs × CN67383, with the status of the locus in each individual verified by progeny test in F. Segregation ratios confirmed the monogenic nature of resistance. Making use of the sequence data of DNA markers and the oat OT3098 v2 genome reference assembly, is located on chromosome 7C. A comparison was also made with the reference consensus map, to which there are more reports of mapped genes to date. The mapping results suggest that is located in the interval 103.8-111.7 cM on this map. No powdery mildew resistance locus has been identified in this region so far, suggesting that CN67383 carries a novel locus offering effective resistance in oat breeding. The information included in the oat genome annotation allowed for the identification of candidate genes in the close region of the marker cluster for . This information may provide an interesting source of further analysis of the pathways of various genes in response to the stress of powdery mildew infection.
鉴定新的、有效的抗病基因是植物育种的一个非常重要的方面。同样重要的是，个别基因座的精确定位，并将其与 DNA 标记进行标记，以便进行标记辅助选择。本研究的目的是鉴定与 powdery mildew 新的有效抗性基因相关的分子标记，并将其定位在燕麦基因组中。在 Fuchs × CN67383 的杂种 167 个 F 个体中进行了分析，通过 F 代的后代测试验证了每个个体的基因座状态。分离比证实了抗性的单基因性质。利用 DNA 标记的序列数据和燕麦 OT3098 v2 基因组参考组装，确定了 位于第 7C 染色体上。还与参考共识图谱进行了比较，到目前为止，该图谱上有更多的基因映射报告。映射结果表明，在该图谱上， 位于 103.8-111.7 cM 区间内。到目前为止，在该区域尚未鉴定出 powdery mildew 抗性基因座，这表明 CN67383 携带一个新的基因座，为燕麦育种提供了有效的抗性。燕麦基因组注释中包含的信息允许在标记簇的附近区域鉴定 的候选基因。这些信息可能为进一步分析各种基因在应对 powdery mildew 感染压力下的途径提供有趣的分析来源。
