Cieplak Magdalena, Nucia Aleksandra, Ociepa Tomasz, Okoń Sylwia
Institute of Plant Genetics, Breeding and Biotechnology, University of Life Science, 20-950 Lublin, Poland.
Plants (Basel). 2022 May 20;11(10):1358. doi: 10.3390/plants11101358.
The structure and dynamics of changes in pathogen populations can be analysed by assessing the level of virulence and genetic diversity. The aim of the present study was to determine the diversity of f. sp. populations. Diversity and virulence of f. sp. was assessed based on 80 single-spore isolates collected in different European countries such as Poland (40 isolates), Germany (10), Finland (10), Czech Republic (10) and Ireland (10) using ISSR () and SCoT () markers. This work demonstrated differences in virulence of f. sp. isolates sampled from different countries. Molecular analysis showed that both systems were useful for assessing genetic diversity, but ISSR markers were superior and generated more polymorphic products, as well as higher PIC and RP values. UPMGA and PCoA divided the isolates into groups corresponding with their geographical origin. In conclusion, the low level of genetic differentiation of the analysed isolates has suggested that the evolution of f. sp. population is slow, and thus the evolutionary potential of the pathogen is low. This work paves the way for future studies on f. sp. population structure and dynamics based on genetic variability.
通过评估毒力水平和遗传多样性，可以分析病原体种群变化的结构和动态。本研究的目的是确定叶锈菌种群的多样性。基于在不同欧洲国家（如波兰（40个分离株）、德国（10个）、芬兰（10个）、捷克共和国（10个）和爱尔兰（10个））收集的80个单孢分离株，使用ISSR（）和SCoT（）标记评估叶锈菌的多样性和毒力。这项工作证明了从不同国家采集的叶锈菌分离株在毒力上存在差异。分子分析表明，这两种系统都有助于评估遗传多样性，但ISSR标记更优，产生了更多多态性产物，以及更高的PIC和RP值。UPMGA和PCoA将分离株分为与其地理来源相对应的组。总之，分析的分离株遗传分化水平较低，这表明叶锈菌种群进化缓慢，因此该病原体的进化潜力较低。这项工作为未来基于遗传变异性研究叶锈菌种群结构和动态铺平了道路。
