Plant Breeding & Acclimatization Institute - National Research Institute, Radzików, 05-870, Błonie, Poland.
J Appl Genet. 2019 Nov;60(3-4):243-254. doi: 10.1007/s13353-019-00505-y. Epub 2019 Jul 17.
Powdery mildew is a barley foliar disease that causes great loss in yield. Because of the limited number of effective resistance genes, efforts to identify new sources of resistance are frequently focused on genetically diversified landraces. The goal of this study was to characterise the powdery mildew resistance gene in barley line 2553-3 selected from the Moroccan landrace. Phytopathological testing against a set of differential pathogen isolates revealed different pattern responses of this gene from those of other known resistance genes. F and F (2553-3 × Manchuria) mapping populations were employed to investigate resistance inheritance. Two approaches were applied for the linkage analysis: in the first approach, 22 resistant and 21 susceptible homozygous F plants genotyped by the DArTseq platform (Diversity Arrays Technology, Pty. Ltd.) were used; in the second, 94 F plants were genotyped by converted DArTseq markers and SSRs. Both analyses delineated a new resistance gene on the short arm of chromosome 2H. The authors propose MlMor as a gene symbol for newly characterized powdery mildew resistance genes in barley line 255-3-3. The results presented herein provide a good foundation for the development of closer linkage markers and MAS breeding.
白粉病是一种大麦叶片病害，可导致产量大幅损失。由于有效抗性基因的数量有限，因此经常致力于鉴定新的抗性来源，这些来源通常来自遗传多样化的地方品种。本研究的目的是对从摩洛哥地方品种中选择的大麦品系 2553-3 中的白粉病抗性基因进行特征描述。针对一组差异病原体分离物进行的植物病理学测试显示，该基因的反应模式与其他已知抗性基因不同。利用 F 和 F (2553-3 × 满洲) 作图群体研究了抗性的遗传。采用了两种方法进行连锁分析：在第一种方法中，使用 DArTseq 平台（Diversity Arrays Technology，Pty. Ltd.）对 22 个抗性和 21 个易感纯合 F 植物进行了基因型分析；在第二种方法中，对 94 个 F 植物进行了转换后的 DArTseq 标记和 SSRs 的基因型分析。两种分析均将一个新的抗性基因定位于 2H 染色体的短臂上。作者提议将 MlMor 作为大麦品系 255-3-3 中白粉病新鉴定的抗性基因的基因符号。本文的结果为开发更紧密的连锁标记和 MAS 育种提供了良好的基础。
