Dietzschold B, Tollis M, Rupprecht C E, Celis E, Koprowski H
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
J Infect Dis. 1987 Nov;156(5):815-22. doi: 10.1093/infdis/156.5.815.
Immunization experiments with vaccines prepared from the PM and ERA strains of rabies virus demonstrated that in mice, only ERA vaccine primes for an anamnestic response to the rabies-related strain Duvenhage (DUV6); in rabbits, both ERA and PM vaccines induced immunologic memory to DUV6 virus. In mice, ERA vaccine, but not an equal concentration of PM vaccine, conferred protection against a lethal challenge infection with DUV6 virus. This result indicated that the protective activity correlated with the vaccine's ability to induce immunologic memory. A vaccine prepared from a sequentially selected, neutralization-resistant, multiple-variant virus conferred protection against challenge with the parental strain, a result indicating that antigenic variation of the glycoprotein may not be the sole factor in determining the relative efficacy of rabies prophylaxis. We found no correlation between titers of neutralizing antibody and mortality rates in mice immunized with purified glycoprotein from these viruses.
用狂犬病病毒PM株和ERA株制备的疫苗进行的免疫实验表明，在小鼠中，只有ERA疫苗能引发对狂犬病相关毒株杜文黑格病毒（DUV6）的回忆反应；在兔子中，ERA疫苗和PM疫苗都能诱导对DUV6病毒的免疫记忆。在小鼠中，ERA疫苗能提供针对DUV6病毒致死性攻击感染的保护，而相同浓度的PM疫苗则不能。这一结果表明，保护活性与疫苗诱导免疫记忆的能力相关。由经序列选择、具有中和抗性的多变异病毒制备的疫苗能提供针对亲代毒株攻击的保护，这一结果表明糖蛋白的抗原变异可能不是决定狂犬病预防相对效果的唯一因素。我们发现，在用这些病毒的纯化糖蛋白免疫的小鼠中，中和抗体滴度与死亡率之间没有相关性。
