Institute of Lung Health and Immunity (LHI), Comprehensive Pneumology Center Munich (CPC-M), Helmholtz Center Munich, Research Center for Environmental Health, 85764 München, Germany.
German Center of Lung Research (DZL), Comprehensive Pneumology Center Munich (CPC-M), 81377 München, Germany.
Int J Mol Sci. 2022 Dec 10;23(24):15666. doi: 10.3390/ijms232415666.
Lung epithelial organoids for the hazard assessment of inhaled nanomaterials offer a promising improvement to in vitro culture systems used so far. Organoids grow in three-dimensional (3D) spheres and can be derived from either induced pluripotent stem cells (iPSC) or primary lung tissue stem cells from either human or mouse. In this perspective we will highlight advantages and disadvantages of traditional culture systems frequently used for testing nanomaterials and compare them to lung epithelial organoids. We also discuss the differences between tissue and iPSC-derived organoids and give an outlook in which direction the whole field could possibly go with these versatile tools.
用于评估吸入性纳米材料危害的肺上皮类器官为迄今为止使用的体外培养系统提供了一个很有前途的改进。类器官在三维（3D）球体中生长，可以源自诱导多能干细胞（iPSC）或源自人或小鼠的原代肺组织干细胞。在本观点中，我们将重点介绍常用于测试纳米材料的传统培养系统的优缺点，并将其与肺上皮类器官进行比较。我们还讨论了组织和 iPSC 衍生类器官之间的差异，并展望了这个多功能工具可能会在哪些方向发展。
