Zhou Hang, Liu Yu, Fan Jiongting, Huang Huajing, Deng Junming, Tan Beiping
College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.
Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang 524088, China.
Metabolites. 2022 Nov 23;12(12):1167. doi: 10.3390/metabo12121167.
A 70-day feeding trial investigated the effects of dietary inclusion of different types of non-starch polysaccharides (NSPs) on gut microbiota and serum metabolome of rainbow trout. Four practical feeds (42% crude protein, 17% crude lipid) were prepared with 8% insoluble NSP (INSP, cellulose), 16.8% soluble NSP (SNSP, composed of 1.12% β-glucan, 1.28% mannan, 4.8% arabinoxylan, and 9.6% pectin), 24.8% NSPs (8% INSP + 16.8% SNSP), or no NSPs inclusion, respectively. Dietary NSPs inclusion had no significant influence on the Shannon, Simpson, ACE, and Chao1 indices of gut microbiota but induced a significant increase in the abundance of and , and a decrease in Firmicutes and Besides, dietary SNSP upregulated the carnitine synthesis metabolic pathway. Our data suggest that dietary NSPs are detrimental to gut microbiota homeostasis and the health of rainbow trout, and dietary SNSP exhibit a stronger ability to interfere with physiological metabolism of rainbow trout than INSP. Therefore, the physiological effects of dietary NSPs, especially SNSP, should be carefully considered when designing the commercial feed formulations of rainbow trout.
一项为期70天的饲养试验研究了日粮中添加不同类型非淀粉多糖（NSPs）对虹鳟肠道微生物群和血清代谢组的影响。制备了四种实用饲料（粗蛋白42%，粗脂肪17%），分别含有8%不溶性NSP（INSP，纤维素）、16.8%可溶性NSP（SNSP，由1.12%β-葡聚糖、1.28%甘露聚糖、4.8%阿拉伯木聚糖和9.6%果胶组成）、24.8% NSPs（8% INSP + 16.8% SNSP）或不添加NSPs。日粮中添加NSPs对肠道微生物群的香农、辛普森、ACE和Chao1指数没有显著影响，但导致[具体菌属1]和[具体菌属2]丰度显著增加，厚壁菌门和[具体菌属3]丰度降低。此外，日粮中的SNSP上调了肉碱合成代谢途径。我们的数据表明，日粮中的NSPs对虹鳟肠道微生物群稳态和健康有害，并且日粮中的SNSP比INSP表现出更强的干扰虹鳟生理代谢的能力。因此，在设计虹鳟商业饲料配方时，应仔细考虑日粮中NSPs，尤其是SNSP的生理效应。
