Li Xuehe, Zhu Dongwenjun, Mao Minling, Wu Jianwei, Yang Qihui, Tan Beiping, Chi Shuyan
College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.
Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China.
Metabolites. 2022 Dec 15;12(12):1268. doi: 10.3390/metabo12121268.
Groupers with an initial body weight of 9.10 ± 0.03 g were selected to investigate whether dietary addition of 0 (G0) and 1800 mg/kg glycerol monolaurate (GML, G1800) could alleviate the oxidative stress response and intestinal flora imbalance after 0, 6, 12, and 24 h of salinity change in grouper. Experimental results show that the dietary addition of GML significantly reduced the liver MDA content and increased the SOD activity of grouper. The gene expression of CAT and SOD increased and then decreased with time after adding 1800 mg/kg GML, and the highest values were significantly higher than those of the control group. Salinity change had a slight effect on the top four intestinal flora composition of grouper at 0, 12, and 24 h, with changes occurring only at 6 h when Cyanobacteria replaced Actinobacteria. The addition of dietary GML slowed down the intestinal flora disorder, inhibited the colonization of harmful bacterium , and promoted the abundance of beneficial bacterium . In conclusion, dietary GML significantly reduced the oxidative damage caused by sudden changes in salinity, improved the antioxidant capacity, and alleviated the intestinal flora imbalance in juvenile grouper.
选取初始体重为9.10±0.03 g的石斑鱼，研究饲料中添加0（G0）和1800 mg/kg月桂酸甘油单酯（GML，G1800）是否能缓解石斑鱼盐度变化0、6、12和24小时后的氧化应激反应和肠道菌群失衡。实验结果表明，饲料中添加GML可显著降低石斑鱼肝脏丙二醛含量并提高其超氧化物歧化酶活性。添加1800 mg/kg GML后，CAT和SOD的基因表达随时间先升高后降低，其最高值显著高于对照组。盐度变化在0、12和24小时对石斑鱼前四大肠道菌群组成影响较小，仅在6小时发生变化，此时蓝细菌取代了放线菌。饲料中添加GML减缓了肠道菌群紊乱，抑制了有害菌的定殖，并促进了有益菌的丰度。总之，饲料中添加GML可显著降低盐度突变引起的氧化损伤，提高抗氧化能力，并缓解幼鱼石斑鱼的肠道菌群失衡。
