Kim Hye-Rim, Han Mi-Suk, Eom Yong-Bin
Department of Medical Sciences, Graduate School, Soonchunhyang University, Asan-si, Chungcheongnam-do 31538 Republic of Korea.
Present Address: Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, 22 Soonchunhyang-ro, Sinchang-myeon, Asan-si, Chungcheongnam-do 31538 Republic of Korea.
Indian J Microbiol. 2022 Sep;62(3):401-410. doi: 10.1007/s12088-022-01020-1. Epub 2022 Apr 18.
has clinical significance due to its etiological role in yersiniosis and gastroenteritis. This study was designed to assess anti-bacterial and anti-biofilm effects of equol on via phenotypic and genetic analyses. To determine its anti-bacterial activity, minimum inhibitory concentrations (MICs) of equol against clinically isolated strains were analyzed. Subsequently, it was confirmed that the sub-MIC value of equol could inhibit biofilm formation and reduce preformed biofilm. Furthermore, it was found that equol could reduce the expression of biofilm-related () gene in . This study also demonstrated that equol not only reduced levels of bacterial motility, but also decreased the expression of a motility-related () gene in . XTT [2,3-bis (2-metoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction analysis revealed that equol attenuated cellular metabolic activities in biofilm. Additionally, changes in biomass and cell density in equol-treated biofilms were visualized using a confocal laser scanning microscope. In conclusion, this study suggests that equol is a potential anti-bacterial and anti-biofilm agent to treat .
由于其在耶尔森氏菌病和肠胃炎中的病因学作用而具有临床意义。本研究旨在通过表型和基因分析评估雌马酚对[具体细菌名称未给出]的抗菌和抗生物膜作用。为确定其抗菌活性，分析了雌马酚对临床分离的[具体细菌名称未给出]菌株的最低抑菌浓度（MIC）。随后，证实了雌马酚的亚MIC值可抑制生物膜形成并减少预先形成的生物膜。此外，发现雌马酚可降低[具体细菌名称未给出]中生物膜相关（[相关基因名称未给出]）基因的表达。本研究还表明，雌马酚不仅降低了细菌的运动水平，还降低了[具体细菌名称未给出]中与运动相关（[相关基因名称未给出]）基因的表达。XTT [2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-2H-四唑-5-甲酰苯胺]还原分析表明，雌马酚减弱了[具体细菌名称未给出]生物膜中的细胞代谢活性。此外，使用共聚焦激光扫描显微镜观察了经雌马酚处理的生物膜中生物量和细胞密度的变化。总之，本研究表明雌马酚是一种治疗[具体细菌名称未给出]的潜在抗菌和抗生物膜剂。
