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头孢磺啶和万古霉素：用于显色大肠菌群琼脂的补充剂，用于检测不同水源中的大肠菌群和大肠杆菌。

Cefsulodin and Vancomycin: A Supplement for Chromogenic Coliform Agar for Detection of and Coliform Bacteria from Different Water Sources.

作者信息

Schalli Michael, Inwinkl Sarah Maria, Platzer Sabine, Baumert Rita, Reinthaler Franz F, Ofner-Kopeinig Petra, Haas Doris

机构信息

Department for Water-Hygiene and Micro-Ecology, D&R Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, 8010 Graz, Austria.

Institute for Medical Informatics, Statistics and Documentation, Medical University of Graz, 8010 Graz, Austria.

出版信息

Microorganisms. 2022 Dec 16;10(12):2499. doi: 10.3390/microorganisms10122499.

DOI:10.3390/microorganisms10122499
PMID:36557752
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9783415/
Abstract

Background microorganism growth on Chromogenic Coliform Agar (CCA) can be challenging. For this reason, a new alternative method with a Cefsulodin/Vancomycin (CV)-supplemented CCA should be developed in this study. CCA supplemented with CV was validated according to ÖNORM EN ISO 16140-4:2021 using water from natural sources in Styria, Austria. Results show that the alternative method using the supplemented CCA has similar values in relation to sensitivity (82.2%), specificity (98.6%) and higher selectivity (59%) compared to the reference method. Repeatability and reproducibility were acceptable for the alternative method and showed similar results with the reference method. The alternative method shows a very low false positive rate and a low false negative rate paired with good performance regarding the inclusion study. The exclusion study shows the advantage of our method by suppressing background microorganisms and facilitating the process of enumeration of and other coliform bacteria on CCA plates. and growth was inhibited using the supplement. To conclude, the coliform CV selective supplement combined with CCA is an appropriate tool for coliform bacteria detection in water samples.

摘要

背景

在显色大肠菌群琼脂（CCA）上培养背景微生物可能具有挑战性。因此，本研究应开发一种新的替代方法，即使用添加头孢磺啶/万古霉素（CV）的CCA。根据ÖNORM EN ISO 16140-4:2021，使用奥地利施蒂利亚州天然水源的水对添加CV的CCA进行了验证。结果表明，与参考方法相比，使用添加CCA的替代方法在灵敏度（82.2%）、特异性（98.6%）和更高的选择性（59%）方面具有相似的值。替代方法的重复性和再现性是可接受的，并且与参考方法显示出相似的结果。替代方法显示出非常低的假阳性率和低假阴性率，并且在纳入研究方面表现良好。排除研究显示了我们方法的优势，即通过抑制背景微生物并促进在CCA平板上对 和其他大肠菌群细菌的计数过程。使用该补充剂可抑制 和 的生长。总之，大肠菌群CV选择性补充剂与CCA相结合是检测水样中大肠菌群细菌的合适工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/29e3d379e859/microorganisms-10-02499-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/ffee3cff6296/microorganisms-10-02499-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/bc9551f5aee7/microorganisms-10-02499-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/7759695abd92/microorganisms-10-02499-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/750548d75b07/microorganisms-10-02499-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/23fa54ac61fa/microorganisms-10-02499-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/ddc35ca84587/microorganisms-10-02499-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/132ccbe138d6/microorganisms-10-02499-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/55e5427d9751/microorganisms-10-02499-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/29e3d379e859/microorganisms-10-02499-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/ffee3cff6296/microorganisms-10-02499-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/bc9551f5aee7/microorganisms-10-02499-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/7759695abd92/microorganisms-10-02499-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/750548d75b07/microorganisms-10-02499-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/23fa54ac61fa/microorganisms-10-02499-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/ddc35ca84587/microorganisms-10-02499-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/132ccbe138d6/microorganisms-10-02499-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/55e5427d9751/microorganisms-10-02499-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3921/9783415/29e3d379e859/microorganisms-10-02499-g009.jpg

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