State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Department of Cardiovascular Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Molecules. 2022 Dec 13;27(24):8860. doi: 10.3390/molecules27248860.
Notoginseng and safflower are commonly used traditional Chinese medicines for benefiting qi and activating blood circulation. A previous study by our group showed that the compatibility of the effective components of total saponins of notoginseng (NS) and total flavonoids of safflower (SF), named NS-SF, had a preventive effect on isoproterenol (ISO)-induced myocardial infarction (MI) in rats. However, the therapeutic effect on MI and the synergistic mechanism of NS-SF are still unclear. Therefore, integrated metabolomics, combined with immunohistochemistry and other pharmacological methods, was used to systematically research the therapeutic effect of NS-SF on MI rats and the synergistic mechanism of NS and SF. Compared to NS and SF, the results demonstrated that NS-SF exhibited a significantly better role in ameliorating myocardial damage, apoptosis, easing oxidative stress and anti-inflammation. NS-SF showed a more significant regulatory effect on metabolites involved in sphingolipid metabolism, glycine, serine, and threonine metabolism, primary bile acid biosynthesis, aminoacyl-tRNA biosynthesis, and tricarboxylic acid cycle, such as sphingosine, lysophosphatidylcholine (18:0), lysophosphatidylethanolamine (22:5/0:0), chenodeoxycholic acid, L-valine, glycine, and succinate, than NS or SF alone, indicating that NS and SF produced a synergistic effect on the treatment of MI. This study will provide a theoretical basis for the clinical development of NS-SF.
三七和红花是常用的补气活血的中药。本课题组前期研究表明，三七总皂苷（NS）与红花总黄酮（SF）有效成分的配伍，即 NS-SF，对异丙肾上腺素（ISO）诱导的大鼠心肌梗死（MI）具有预防作用。然而，NS-SF 对 MI 的治疗作用及协同机制尚不清楚。因此，本研究采用整合代谢组学方法，结合免疫组化等药理学方法，系统研究 NS-SF 对 MI 大鼠的治疗作用及 NS 和 SF 的协同机制。与 NS 和 SF 相比，结果表明 NS-SF 对改善心肌损伤、细胞凋亡、减轻氧化应激和抗炎具有更好的作用。NS-SF 对参与鞘脂代谢、甘氨酸、丝氨酸和苏氨酸代谢、初级胆汁酸生物合成、氨酰-tRNA 生物合成和三羧酸循环的代谢物，如神经鞘氨醇、溶血磷脂酰胆碱（18:0）、溶血磷脂酰乙醇胺（22:5/0:0）、鹅去氧胆酸、L-缬氨酸、甘氨酸和琥珀酸，具有更显著的调节作用，优于 NS 或 SF 单独使用，表明 NS 和 SF 在治疗 MI 方面具有协同作用。本研究为 NS-SF 的临床开发提供了理论依据。
