迷迭香精油和姜黄素的协同保肝活性：MEK/ERK 通路的作用。

The Synergistic Hepatoprotective Activity of Rosemary Essential Oil and Curcumin: The Role of the MEK/ERK Pathway.

机构信息

Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa 31982, Saudi Arabia.

Department of Pharmacognosy, Faculty of Pharmacy, University of Zagazig, Zagazig 44519, Egypt.

出版信息

Molecules. 2022 Dec 15;27(24):8910. doi: 10.3390/molecules27248910.

DOI:10.3390/molecules27248910

PMID:36558044

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9781795/

Abstract

BACKGROUND

Curcumin is a natural product obtained from the rhizome of . Rosemary () is a medicinal and aromatic plant that is widely spread in the Mediterranean region. Both Curcumin and rosemary essential oil are natural products of high medicinal and pharmacological significance. The hepatoprotective effect of both natural products is well-established; however, the mechanism of such action is not fully understood. Thus, this study is an attempt to explore the hepatoprotective mechanism of action of these remedies through their effect on MEK and ERK proteins. Furthermore, the effect of rosemary essential oil on the plasma concentration of curcumin has been scrutinized.

MATERIALS AND METHODS

The major constituents of REO were qualitatively and quantitatively determined by GC/MS and GC/FID, respectively. Curcumin and rosemary essential oil were given to mice in a pre-treatment model, followed by induction of liver injury through a high dose of paracetamol. Serum liver enzymes, lipid peroxidation, antioxidant activities, the inflammatory and apoptotic biomarkers, as well as the MEK and ERK portions, were verified. The plasma levels of curcumin were determined in the presence and absence of rosemary essential oil.

RESULTS

The major constituents of REO were 1,8-cineole (51.52%), camphor (10.52%), and α-pinene (8.41%). The results revealed a superior hepatoprotective activity of the combination when compared to each natural product alone, as demonstrated by the lowered liver enzymes, lipid peroxidation, mitigated inflammatory and apoptotic biomarkers, and enhanced antioxidant activities. Furthermore, the combination induced the overexpression of MEK and ERK proteins, providing evidence for the involvement of this cascade in the hepatoprotective activity of such natural products. The administration of rosemary essential oil with curcumin enhanced the curcuminoid plasma level.

CONCLUSION

The co-administration of both curcumin and rosemary essential oil together enhanced both their hepatoprotective activity and the level of curcumin in plasma, indicating a synergistic activity between both natural products.

摘要

背景

姜黄素是一种从姜黄根茎中提取的天然产物。迷迭香是一种药用和芳香植物，广泛分布在地中海地区。姜黄素和迷迭香油都是具有高度药用和药理学意义的天然产物。这两种天然产物的保肝作用已得到证实；然而，其作用机制尚不完全清楚。因此，本研究试图通过研究它们对 MEK 和 ERK 蛋白的影响来探索这些药物的保肝作用机制。此外，还研究了迷迭香油对姜黄素血浆浓度的影响。

材料和方法

通过 GC/MS 和 GC/FID 分别对 REO 的主要成分进行定性和定量分析。在预先处理模型中，给小鼠给予姜黄素和迷迭香油，然后用大剂量对乙酰氨基酚诱导肝损伤。验证血清肝酶、脂质过氧化、抗氧化活性、炎症和凋亡生物标志物以及 MEK 和 ERK 部分。在存在和不存在迷迭香油的情况下测定血浆中姜黄素的水平。

结果

REO 的主要成分是 1,8-桉叶素（51.52%）、樟脑（10.52%）和α-蒎烯（8.41%）。结果表明，与单独使用每种天然产物相比，组合具有更好的保肝活性，表现为降低肝酶、脂质过氧化、减轻炎症和凋亡生物标志物以及增强抗氧化活性。此外，该组合诱导 MEK 和 ERK 蛋白的过度表达，为该级联参与这些天然产物的保肝活性提供了证据。姜黄素与迷迭香油同时给药可提高姜黄素类的血浆水平。