Department of Quality Assessment and Processing of Animal Products, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
The Institute of Human Nutrition Science and Agricultural, University College of Applied Sciences in Chełm, Pocztowa 54, 22-100 Chełm, Poland.
Molecules. 2022 Dec 16;27(24):8973. doi: 10.3390/molecules27248973.
The chemical composition, content of cholesterol, retinol and α-tocopherol, and the total antioxidant capacity of different tissues from wild beavers were investigated. The total phenolic contents and free radical scavenging activity (DPPH and ABTS assays) were analysed spectrophotometrically, and fat-soluble vitamins were quantified using high-performance liquid chromatography. The type of tissue (skeletal muscle from loin and hind leg vs. adipose tissue from subcutaneous fat and tail) significantly affected content of all chemical components. The concentration of cholesterol was not related to total fat content. The retinol and α-tocopherol contents (µg/100 g) were significantly higher in the tail (13.0 and 391.2) and subcutaneous fat (12.2 and 371.3) compared to skeletal muscles (as an average 9.1 and 361.4). Among all tissues the tail showed significantly the highest values of DPPH (3.07 mM TE/100 g), ABTS (3.33 mM TE/100 g), and total phenolics (TPC, 543.7 mg GAE/100 g). The concentration of retinol was positively correlated with α-tocopherol (0.748, p < 0.001), and both vitamins were very strongly correlated with DPPH (0.858 and 0.886, p < 0.001), ABTS (0.894 and 0.851, p < 0.001), and TPC (0.666 and 0.913, p < 0.001). The principal component analysis proved that moisture, ash, and protein contents were representative for skeletal muscles, whereas, retinol, α-tocopherol, ABTS and DPPH accurately described the antioxidant capacity of tissue from the tail.
研究了野生海狸不同组织的化学成分、胆固醇、视黄醇和 α-生育酚含量以及总抗氧化能力。使用分光光度法分析了总酚含量和自由基清除活性（DPPH 和 ABTS 测定），并使用高效液相色谱法定量了脂溶性维生素。组织类型（腰和后腿的骨骼肌与皮下脂肪和尾巴的脂肪组织）显著影响所有化学成分的含量。胆固醇浓度与总脂肪含量无关。视黄醇和 α-生育酚含量（µg/100 g）在尾巴（分别为 13.0 和 391.2）和皮下脂肪（分别为 12.2 和 371.3）中明显高于骨骼肌（平均为 9.1 和 361.4）。在所有组织中，尾巴的 DPPH（3.07 mM TE/100 g）、ABTS（3.33 mM TE/100 g）和总酚（TPC，543.7 mg GAE/100 g）值最高。视黄醇浓度与 α-生育酚呈正相关（0.748，p < 0.001），这两种维生素与 DPPH（0.858 和 0.886，p < 0.001）、ABTS（0.894 和 0.851，p < 0.001）和 TPC（0.666 和 0.913，p < 0.001）非常强相关。主成分分析证明，水分、灰分和蛋白质含量是骨骼肌的代表性指标，而视黄醇、α-生育酚、ABTS 和 DPPH 准确描述了尾巴组织的抗氧化能力。
