Vieira Daniela, Allard Jérôme, Taylor Kathleen, Harvey Edward J, Merle Geraldine
Department of Experimental Surgery, Faculty of Medicine, McGill University, 1650 Cedar Avenue, A7-117, Montreal, QC H3G 1A4, Canada.
Department of Chemical Engineering, Polytechnique Montreal, J.-A.-Bombardier Building, Office 2067, Montreal, QC H3C 3A7, Canada.
Nanomaterials (Basel). 2022 Dec 13;12(24):4431. doi: 10.3390/nano12244431.
Recently, the abnormal level of zinc emerged as a powerful indicator or risk factor for metabolic, endocrine, neurodegenerative and cardiovascular diseases, including cancer. Electrochemical detection has been explored to quantify zinc in a precise, rapid, and non-expensive way; however, most of the current electrochemical systems lack in specificity. In this work we studied a highly selective and sensitive electrochemical method to detect quickly and reliably free zinc ions (Zn). The surface of the working electrode was modified with zincon electropolymerized on carbon nanotube (CNT) to enable the binding of zinc in complex body fluids. After being physicochemically characterized, the performances of the zincon-CNT complex was electrochemically assessed. Square Wave Voltammetry (SWV) was used to determine the calibration curve and the linear range of zinc quantification in artificial saliva and urine. This zincon- CNT system could specifically quantify mobile Zn in salivary and urinary matrices with a sensitivity of ~100 ng·mL and a limit of detection (LOD) of ~20 ng·mL. Zincon-modified CNT presented as a desirable candidate for the detection and quantification of free zinc in easily body fluids that potentially can become a diagnostic non-invasive testing platform.
最近，锌水平异常已成为包括癌症在内的代谢、内分泌、神经退行性和心血管疾病的一个有力指标或风险因素。人们已探索采用电化学检测方法以精确、快速且经济的方式对锌进行定量；然而，当前大多数电化学系统缺乏特异性。在这项工作中，我们研究了一种高选择性和灵敏性的电化学方法，用于快速且可靠地检测游离锌离子（Zn）。工作电极表面用在碳纳米管（CNT）上电聚合的锌试剂进行修饰，以使锌能够在复杂的体液中结合。在进行物理化学表征后，对锌试剂 - CNT 复合物的性能进行了电化学评估。采用方波伏安法（SWV）来确定人工唾液和尿液中锌定量的校准曲线和线性范围。这种锌试剂 - CNT 系统能够特异性地定量唾液和尿液基质中的游离锌，灵敏度约为 100 ng·mL，检测限（LOD）约为 20 ng·mL。锌试剂修饰的 CNT 是检测和定量易获取体液中游离锌的理想候选物，有望成为一种非侵入性诊断检测平台。
