Hickey G J, Hansel W
Department of Physiology, New York State College of Veterinary Medicine, Ithaca.
J Reprod Fertil. 1987 Jul;80(2):569-76. doi: 10.1530/jrf.0.0800569.
Two culture systems for maintenance of Day 13-18 conceptus tissue were developed. Harvested culture media were assayed for luteotrophic activity by determining their ability to stimulate progesterone synthesis by dispersed bovine luteal cells. Significant luteotrophic activity was found in 80% of the 31 tissue culture media studied. A series of experiments carried out to determine the nature of the luteotrophic activity indicate that it is a small (Mr less than 10,000), heat-labile, lipid-soluble substance that is adsorbed by dextran-coated charcoal. The nature and activity of this factor, together with its synthesis by the early bovine conceptus, suggest that it may have a significant role in stimulating progesterone synthesis by the corpus luteum during early pregnancy.
开发了两种用于维持第13 - 18天孕体组织的培养系统。通过测定收获的培养基刺激分散的牛黄体细胞合成孕酮的能力，来检测其黄体营养活性。在所研究的31种组织培养基中，80%发现有显著的黄体营养活性。为确定黄体营养活性的性质而进行的一系列实验表明，它是一种小分子物质（分子量小于10,000），对热不稳定，脂溶性，可被葡聚糖包被的活性炭吸附。该因子的性质和活性，以及其由早期牛孕体合成，表明它可能在妊娠早期刺激黄体合成孕酮方面发挥重要作用。
