Chen Lu, Zhang Weiwei, Xie Ping, Ji Jiangdong, Qian Huiming, Yuan Songtao, Liu Qinghuai, Hu Zizhong
Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Xuzhou Key Laboratory of Ophthalmology, The Municipal Affiliated Hospital of Xuzhou Medical University, Eye Institute of Xuzhou, Xuzhou, Jiangsu 221000, China.
J Ophthalmol. 2021 Jul 13;2021:9947250. doi: 10.1155/2021/9947250. eCollection 2021.
Idiopathic epiretinal membrane (iERM) or idiopathic macular hole (iMH) is frequently used as a "healthy" control in comparison of vitreous cytokines with other vitreoretinal diseases. This study aimed to investigate if there is a difference in vitreal cytokines expression between patients with iERM and iMH.
In this prospective study, all subjects received standard pars plana vitrectomy surgery, and 0.5 ml of native vitreous sample was extracted during the vitrectomy. Luminex technology and enzyme-linked immunosorbent assay were used to profile the concentration of 52 classic angiogenic, inflammatory, and profibrotic cytokines and chemokines. Statistical analyses were performed by the Mann-Whitney U test, followed by multiple comparisons by the Bonferroni correction.
Vitreal samples from 13 iERM and 24 iMH were studied. Of the 52 tested cytokines, 41 were similar in expression, and 5 were under the detection limit, while 6 cytokines (MMP-8, Eotaxin, MIP-1a, RANTES, TGF-2, and IL-4) were differently expressed between two groups ( < 0.05). Nevertheless, these significances disappeared after the adjustment of Bonferroni correction.
The tested cytokines showed similar expression between iERM and iMH patients. This indicates that eyes with iERM or iMH can be together served as "healthy" controls.
特发性视网膜前膜（iERM）或特发性黄斑裂孔（iMH）常被用作与其他玻璃体视网膜疾病比较玻璃体细胞因子时的“健康”对照。本研究旨在调查iERM和iMH患者玻璃体细胞因子表达是否存在差异。
在这项前瞻性研究中，所有受试者均接受标准的玻璃体切割手术，在玻璃体切割过程中提取0.5 ml天然玻璃体样本。采用Luminex技术和酶联免疫吸附测定法分析52种经典血管生成、炎症和促纤维化细胞因子及趋化因子的浓度。采用Mann-Whitney U检验进行统计分析，随后采用Bonferroni校正进行多重比较。
研究了13例iERM和24例iMH的玻璃体样本。在52种检测的细胞因子中，41种表达相似，5种低于检测限，而6种细胞因子（基质金属蛋白酶-8、嗜酸性粒细胞趋化因子、巨噬细胞炎性蛋白-1α、调节激活正常T细胞表达和分泌因子、转化生长因子-β2和白细胞介素-4）在两组间表达不同（P<0.05）。然而，经Bonferroni校正调整后，这些显著性消失。
所检测的细胞因子在iERM和iMH患者中表达相似。这表明患有iERM或iMH的眼睛可共同用作“健康”对照。
