Liang Zhengwei, Liu Kunyi, Li Ruoyu, Ma Baiping, Zheng Wei, Yang Shengchao, Zhang Guanghui, Zhao Yinhe, Chen Junwen, Zhao Ming
College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, China.
Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, China.
Front Nutr. 2022 Dec 7;9:1058639. doi: 10.3389/fnut.2022.1058639. eCollection 2022.
Radix Notoginseng, one of the most famous Chinese traditional medicines, is the dried root of (Araliaceae). Stems and leaves of (SLPN) are rich in secondary metabolites and nutrients, and authorized as a food resource, however, its utilization needs further research.
A SLPN-instant beverage was manufactured from SLPN through optimization by response surface design with 21-fold of 48.50% ethanol for 39 h, and this extraction was repeated twice; the extraction solution was concentrated to 1/3 volume using a vacuum rotatory evaporator at 45°C, and then spray dried at 110°C. Nutritional components including 14 amino acids, ten mineral elements, 15 vitamins were detected in the SLPN-instant beverage; forty-three triterpenoid saponins, e.g., ginsenoside La, ginsenoside Rb3, notoginsenoside R1, and two flavonoid glycosides, as well as dencichine were identified by UPLC-MS.
The extraction rate of SLPN-instant beverage was 37.89 ± 0.02%. The majority nutrients were Gly (2.10 ± 0.63 mg/g), His (1.23 ± 0.07 mg/g), α-VE (18.89 ± 1.87 μg/g), β-VE (17.53 ± 1.98 μg/g), potassium (49.26 ± 2.70 mg/g), calcium (6.73 ± 0.27 mg/g). The total saponin of the SLPN-instant beverage was 403.05 ± 34.98 mg/g, majority was notoginsenoside Fd and with contents of 227 ± 2.02 mg/g. In addition, catechin and γ-aminobutyric acid were detected with levels of 24.57 ± 0.21 mg/g and 7.50 ± 1.85 mg/g, respectively. The SLPN-instant beverage showed good antioxidant activities with half maximal inhibitory concentration (IC) for scavenging hydroxyl (OH) radicals, superoxide anion (O) radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) radicals were 0.1954, 0.2314, 0.4083, and 0.3874 mg/mL, respectively.
We optimized an analytical method for in depth analysis of the newly authorized food resource SLPN. Together, an instant beverage with antioxidant activity, rich in nutrients and secondary metabolites, was manufactured from SLPN, which may improve the utilization of SLPN.
三七是最著名的传统中药之一，为五加科植物三七的干燥根。三七茎叶富含次生代谢产物和营养成分，已被批准为食品资源，但其利用仍需进一步研究。
通过响应面设计优化三七茎叶的提取工艺，以21倍体积的48.50%乙醇提取39小时，重复提取两次；提取液在45℃下用真空旋转蒸发仪浓缩至1/3体积，然后在110℃下进行喷雾干燥。检测三七茎叶速溶饮料中的14种氨基酸、10种矿物质元素、15种维生素等营养成分；采用超高效液相色谱-质谱联用仪鉴定了43种三萜皂苷（如人参皂苷La、人参皂苷Rb3、三七皂苷R1）、两种黄酮苷以及三七素。
三七茎叶速溶饮料的提取率为37.89±0.02%。主要营养成分有甘氨酸（2.10±0.63毫克/克）、组氨酸（1.23±0.07毫克/克）、α-生育酚（18.89±1.87微克/克）、β-生育酚（17.53±1.98微克/克）、钾（49.26±2.70毫克/克）、钙（6.73±0.27毫克/克）。三七茎叶速溶饮料总皂苷含量为403.05±34.98毫克/克，主要为三七皂苷Fd，含量为227±2.02毫克/克。此外，还检测到儿茶素和γ-氨基丁酸，含量分别为24.57±0.21毫克/克和7.50±1.85毫克/克。三七茎叶速溶饮料对清除羟基（OH）自由基、超氧阴离子（O）自由基、1,1-二苯基-2-苦基肼（DPPH）自由基和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS+）自由基的半数抑制浓度（IC）分别为0.1954、0.2314、0.4083和0.3874毫克/毫升，具有良好的抗氧化活性。
我们优化了一种用于深入分析新批准食品资源三七茎叶的分析方法。同时，以三七茎叶为原料制备了具有抗氧化活性、富含营养成分和次生代谢产物的速溶饮料，这可能提高三七茎叶的利用率。
